American Journal of Respiratory Cell and Molecular Biology

Acquired cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction may contribute to chronic obstructive pulmonary disease pathogenesis and is a potential therapeutic target. We sought to determine the acute effects of cigarette smoke on ion transport and the mucociliary transport apparatus, their mechanistic basis, and whether deleterious effects could be reversed with the CFTR potentiator ivacaftor (VX-770). Primary human bronchial epithelial (HBE) cells and human bronchi were exposed to cigarette smoke extract (CSE) and/or ivacaftor. CFTR function and expression were measured in Ussing chambers and by surface biotinylation. CSE-derived acrolein modifications on CFTR were determined by mass spectroscopic analysis of purified protein, and the functional microanatomy of the airway epithelia was measured by 1-μm resolution optical coherence tomography. CSE reduced CFTR-dependent current in HBE cells (P < 0.05) and human bronchi (P < 0.05) within minutes of exposure. The mechanism involved CSE-induced reduction of CFTR gating, decreasing CFTR open-channel probability by approximately 75% immediately after exposure (P < 0.05), whereas surface CFTR expression was partially reduced with chronic exposure, but was stable acutely. CSE treatment of purified CFTR resulted in acrolein modifications on lysine and cysteine residues that likely disrupt CFTR gating. In primary HBE cells, CSE reduced airway surface liquid depth (P < 0.05) and ciliary beat frequency (P < 0.05) within 60 minutes that was restored by coadministration with ivacaftor (P < 0.005). Cigarette smoking transmits acute reductions in CFTR activity, adversely affecting the airway surface. These effects are reversible by a CFTR potentiator in vitro, representing a potential therapeutic strategy in patients with chronic obstructive pulmonary disease with chronic bronchitis.

Cigarette smoking can cause acquired cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction, but its impact on the mucociliary transport (MCT) apparatus with and without potentiation of CFTR is poorly characterized. Cigarette smoke extract caused an acute reduction in CFTR channel gating and conferred a severe reduction in MCT in vitro. The CFTR potentiator, ivacaftor, reversed these abnormalities, even with chronic administration.

New therapies are needed for the treatment of chronic obstructive pulmonary disease (COPD), which accounts for over $40 billion in annual U.S. healthcare costs (1), and recently surpassed stroke as the third leading cause of death in the United States (2). Smoking is the primary risk factor for the disease, and is responsible for nearly 90% of COPD-related deaths (3). Cigarette smoke may contribute to the pathogenesis of COPD by altering the function of cystic fibrosis (CF) transmembrane conductance regulator (CFTR) (48), a protein that functions as an anion channel at the apical surface of airway epithelia. Mutation of the CFTR gene is responsible for CF, an airway disease that shares a similar phenotype with that of patients with COPD that exhibit chronic bronchitis. Indeed, cigarette smoke has been observed to elicit a phenotype characteristic of “acquired CFTR dysfunction,” including reduced CFTR activity, enhanced mucus production, and a pronounced impairment of mucociliary transport (MCT) (8). Patients with COPD exhibit reduced CFTR activity, as detected in the upper airways (8), lower airways (9), sweat glands (7, 10), and intestine (7). Furthermore, CFTR dysfunction is independently associated with chronic bronchitis and dyspnea (710), and can persist despite smoking cessation (7).

We have previously shown that acquired CFTR dysfunction phenotype can be reversed by the CFTR potentiator, ivacaftor, in vitro by activating wild-type CFTR-dependent short-circuit current (ISC) after chronic exposure (8). The mechanisms by which CFTR function is reduced by smoking continues to emerge, and its molecular basis is not known. CFTR gating can be acutely blocked by acrolein (7), an important cigarette smoke constituent; this latter mechanism may be amenable to CFTR activator therapy. Other in vitro studies in epithelial monolayers showed that, in addition to ion channel dysfunction, cigarette smoke or cigarette smoke extract (CSE) also reduced CFTR mRNA and protein expression (5, 6, 11, 12). Reduction of membrane-bound CFTR levels via mechanisms that do not rely on mRNA expression have also been shown, including CFTR internalization sufficient to impact airway hydration (4). These mechanisms could have important implications as to whether augmentation of CFTR activity by a potentiator, which activates ion channel gating, is a viable approach.

Here, we evaluated the effects of cigarette smoke on CFTR function, focusing on acute-acting pathways that could lend insight into molecular mechanisms, and established that a large negative effect on channel gating of excised patches occurs almost immediately upon exposure that was associated with direct modifications to the protein itself, making CFTR a good candidate for functional rescue by potentiators. Further studies demonstrated that administration of ivacaftor partially restores epithelial function acutely, substantiating its potential as a novel therapy for COPD.

CFTR Function in Primary Human Airway Epithelial Cells and Tissues

Human sample use was approved by the Institutional Review Boards at University of Alabama Birmingham (no. X080625002) and Massachusetts General Hospital (no. 2008P000178). Primary human bronchial epithelial (HBE) cells were derived from lung explants after written informed consent from subjects with and those without CF by methods described previously (13, 14). Primary human airway cells were grown at an air–liquid interface until terminally differentiated. ISC was measured under voltage clamp conditions using MC8 clamps and P2300 Ussing chambers (Physiologic Instruments, San Diego, CA) (14). Stable plateaus of at least 10 minutes were achieved before addition of each reagent. ISC measurements in human airway explants were conducted after dissection of mucosal layer, and performed as described in cells, with the exception of use of P2307 tissue mounts.

CFTR Western Blots and Biotinylation Studies

For Western blotting, cell lysates were normalized for protein content, electrophoresed under SDS-PAGE conditions, and CFTR detected by chemiluminescence using 10B6.2 anti-CFTR antibody (Millipore Corp., Billerica, MA) (15). Data were normalized to tubulin loading control and performed on three occasions for quantification. Surface CFTR was quantified by biotinylation studies, as previously described (8). Briefly, cells were exposed to biotin for 45 minutes, lysed by sonication, and then precipitated with NeutrAvidin beads (Thermo Fisher Scientific, Waltham, MA) followed by CFTR detection, as described previously here. For biochemical detection of acrolein modification, after acute exposure of 16HBE cells, biotin hydrazide was used to label acrolein-modified protein fraction of cell lysates, followed by immunoprecipitation of CFTR and subsequent detection of acrolein by Western blot analysis against streptavadin.

Macropatch and Single-Channel Recordings

Conventional patch clamp techniques were used to record single-channel currents from inside-out patches from HEK293 cells expressing wild-type CFTR. Pipettes were partially filled with a solution containing 150 mM CsCl, 1 mM CaCl2, 1 mM MgCl2, and 10 mM Hepes (pH 7.2). Single-channel currents were obtained using an Axopatch 200B patch clamp amplifier (Axon Instruments [AI], Molecular Devices, Sunnyvale, CA) controlled by Clampex software (pClamp 10; Axon Instruments) and digitized (Digidata 1440A interface; AI) at a sampling frequency of 1 kHz. After excision, patches were perfused at pH 7.2 with 150 mM CsCl, 1 mM CaCl2, 1 mM MgCl2, and 10 mM Hepes containing 1 mM MgATP and 50 U/ml protein kinase catalytic subunit (Promega, Madison, WI) with or without 1% CSE. Appropriate vehicle (DMSO) controls were used for comparison. Clampfit software (AI) was used to calculate open probability. For display, recordings were filtered at 500 Hz.

Detection of Acrolein Modifications on Purified CFTR Protein Using Mass Spectroscopy

A stable mammalian cell expression system consisting of HEK293 cells was used to express D-165 human CFTR construct (SUMO*-huCFTR.901FLAG-GFP*). This expression system is capable of yielding sufficient quality and quantity of human CFTR protein suitable for biophysical and structural studies. The methods involved in design and development of this expression system, as well as the biophysical and functional characterization of CFTR, are described in an earlier report (16).

The 1-μm Resolution Optical Coherence Tomography Measurements of Functional Microanatomy

The 1-μm resolution optical coherence tomography (μOCT), image acquisition, and processing have been described previously (17). In the current study, cross-sectional images of 0.5 mm × 0.5 mm (x, z) were acquired at a spatial resolution of 2 μm × 2 μm × 1 μm (x, y, z) and a speed of 32–128 frames/s. The methods used to measure airway surface liquid (ASL) depth and ciliary beat frequency from the μOCT data have been described previously (18).


CSE was prepared by bubbling smoke from one 3R4F Research cigarette (University of Kentucky, Lexington, KY) through 1 ml of DMSO at 2 s/10 ml puff, 10 puffs over 3 minutes to make 100% extract (optical density at 320 nm = 0.3 at 100-fold dilution), then applied to cells diluted in media at the percentages shown, an approach similar to the one used extensively in our previous studies in vitro (7, 8, 1921). Ivacaftor was obtained from Selleckchem (Houston, TX). Other agents were purchased commercially from Sigma-Aldrich (St. Louis, MO).

Statistical Analysis

For quantitative measures, including in vitro studies and potential difference testing, descriptive statistics were computed and means compared using Student’s t test or ANOVA, as appropriate. Statistical tests were two sided and performed at a 5% significance level using GraphPad Prism (GraphPad Software, Inc., La Jolla, CA). Error bars designate SEM unless indicated otherwise.

Cigarette Smoke Acutely Blocks Wild-Type CFTR Activity in Primary Human Airway Cells and in Intact Human Trachea Mucosa

We have previously demonstrated that cigarette smoke exposure, either as CSE (8) or gas whole cigarette smoke (20), inhibits CFTR function when the epithelia are exposed for 24 hours or more; other laboratories have shown similar findings (4, 5, 11). To test whether acute inhibition (i.e., 20 min or less) of CFTR currents also occurred, which would suggest acute-acting mechanisms, primary human airway cells underwent CFTR activation with forskolin (10 μM) in the setting of amiloride (100 μM) to block ENaC channels, followed by addition of CSE (2%) or DMSO vehicle control. As shown in representative ISC tracings, significant (∼50%) CFTR inhibition occurred immediately, and was sensitive to CFTRInh-172 (Figure 1A). Summary data showed that CSE inhibited ISC by 28.5 (±1.8) μA/cm2 (P < 0.0001), which represented a substantial fraction of forskolin-induced currents (Figure 1B). Complementary studies that examined inhibition of ISC at the outset of the ISC trace, before addition of amiloride or forskolin, also showed an immediate reduction in ISC (Figure 1C) that was sensitive to CFTRInh-172, indicating that even basal levels of CFTR anion transport can be altered by addition of cigarette smoke. As expected, the magnitude of ISC inhibition was less (compare Figures 1B and 1C), because CFTR was not yet fully activated before CSE addition.

We next tested whether this degree of current inhibition was also observed in excised human airway explants to evaluate whether these findings occurred in native tissue. As shown in representative tracings, ISC was again acutely inhibited by addition of CSE after CFTR activation with forskolin (Figure 1D). Summary data indicated a 7.0 (±0.7) μA/cm2 reduction in ISC as compared with a 0.4 (±0.01) μA/cm2 stimulus with forskolin (Figure 1E, abrogating ∼50% of CFTR stimulated currents; P < 0.005).

To determine the kinetics of reduced CFTR expression under these conditions, we evaluated the effect of CSE on total and cell surface CFTR expression in primary HBE cells at two time points: 20 minutes, representing acute exposure, and 24 hours, representing more chronic exposures studied previously (8). As shown in Figure 2, CSE reduced total CFTR expression and cell surface CFTR expression, but only after chronic exposure; in contrast, 20-minute exposures did not significantly alter either total CFTR expression or cell surface expression, although a trend emerged with the latter, probably reflecting initiation of endocytic recycling (4). In total, these findings indicate that CSE induces an acute blockade of CFTR activity that occurs in very short timeframes in both cells and tissues, and cannot be completely explained by a reduction in cell surface CFTR expression.

Effects of Cigarette Smoke on CFTR Channel Gating

Acute blockade of CFTR-dependent currents that were not dependent on CFTR expression levels suggested that acute effects on CFTR channel gating may be occurring, as also suggested in a report by Moran and colleagues (22). To confirm, we conducted whole-cell and membrane patch clamp studies on HEK293 cells expressing wild-type CFTR. Upon exposure to CSE (1%), there was an immediate reduction in CFTR currents (Figures 3A and 3B). Single-channel conductance studies demonstrated a deleterious effect of CSE on CFTR open-channel probability, a fundamental measure of channel gating, which was reduced by 59% (Figures 3C and 3D).

Cigarette Smoke and Acrolein Covalently Modify CFTR

The acute effects of CSE on CFTR in membrane patches suggested that CFTR itself may be directly impacted by inhalational exposure. Acrolein is one active component of cigarette smoke that we have previously shown affects CFTR gating in vitro and channel function in vivo (7). Given that acrolein can rapidly modify protein structures (23), and its effects on CFTR activity occurred instantaneously, whereas even 24-hour acrolein exposure did not alter CFTR protein expression (7), we postulated that CSE and acrolein may directly modify CFTR to reduce its ion transport function. To test this, purified recombinant CFTR protein was treated with CSE (1%) acutely and compared with protein samples similarly treated with acrolein (1 μM) and DMSO vehicle. Using nano–liquid chromatography tandem mass spectrometry, acrolein modifications can be detected on protein residues. The unsaturated β-carbon of acrolein readily reacts with cysteine groups to form thioether adducts and the carbonyl carbon irreversibly interacts with the primary amine of lysine residues to form Michael adducts (2426); in addition, acrolein may also react with the imidazole group of histidine and the guanidino group of arginine. Trypsin-digested CFTR protein reacted with CSE or acrolein yielded eight identical peptides with detectable acrolein modifications. The spectra of representative peptides from purified CFTR contained modified cysteine and lysine residues (Figures 4A and 4B). The amino acid sequence and the change in predicted mass-to-charge ratio (m/z) were consistent with the presence of acrolein adducted via Michael and Schiff base additions to CFTR. Mass spectrometry analysis detected mass changes of 56.02 Da, indicating Michael additions by acrolein on several cysteine and lysine residues (Figures 4B and 4C) and a single mass change of 38.01 Da, suggesting Schiff base addition on Cys524 (Figure 4A).

To confirm acrolein modifications to CFTR biochemically, we labeled protein lysates isolated from acrolein-exposed 16HBE cells with biotin hydrazide, which specifically binds amino acids modified with acrolein, purified with avidin chromatography, and then immunoprecipitated for CFTR, and detected acrolein modifications with streptavidin–horseradish peroxidase. Results showed clear acrolein modifications to isolated CFTR protein (Figure 4D). Moreover, we have previously shown that cigarette smoking causes acrolein modifications in a previous report (7). In aggregate, these findings confirm that CSE and acrolein modify the CFTR protein itself, and does so in a rapid fashion. We postulate that these covalent modifications to CFTR may contribute to altered structure that predisposes CFTR to decreased ion transport, and may also contribute to its instability at the cell surface.

The Effects of Acute and Chronic Cigarette Smoke on the Functional Microanatomy of Primary HBE Cells

Given the deleterious effects of cigarette smoke on CFTR-dependent anion transport, we next examined the impact of acute smoke exposure on the airway surface, which is severely affected in COPD (27). For this task, we used μOCT imaging, because it provides an integrated measure of multiple aspects of the functional microanatomy of the airway surface without additions of contrast dye or microparticles (18, 28), and used mucosal CSE addition, as expected to occur in vivo. CSE had a deleterious effect on ASL depth (Figures 5A and 5B), and ciliary beating (Figures 5A and 5C) that was present as early as 20 minutes, indicative of the importance and impact of acute-acting pathways on the MCT apparatus. These results demonstrated that reduced CFTR-mediated anion secretion, together with excessive mucus expression (8, 29), rapidly and severely inhibits the mucociliary clearance (MCC) apparatus of airway monolayers.

Ivacaftor Reverses Acquired CFTR Abnormalities and Augments Airway Surface Hydration and Ciliary Beating In Vitro

Although CFTR potentiators were originally developed to restore activity to mutant CFTR localized to the cell surface, some CFTR potentiators, including the clinically approved potentiator, ivacaftor, also augment wild-type CFTR function in human airway epithelia (13, 30) (as opposed to F508 del CFTR that does not reside at the cell surface and exhibits little response to ivacaftor [31]). In excised patches after inhibition of CFTR by CSE, ivacaftor significantly augmented CFTR open channel probability (Figures 3C and 3D). These results indicate that CSE modified CFTR, even in excised patch where the influence of other proteins are minimal, are responsive to CFTR potentiators, and thus confirmed the relevance of this mechanism for the rescue of CFTR function.

To test whether potentiated CFTR channel gating could also improve epithelial function, we again turned to μOCT imaging of primary HBE monolayers, conducted in cells simultaneously exposed to CSE and ivacaftor and monitored acutely. Ivacaftor robustly increased ASL depth in CSE-exposed cells within 60 minutes (Figures 5A and 5B), which was accompanied by a trend toward improved ciliary beating at 20 minutes, but not 60 minutes (Figures 5A and 5C). Ivacaftor treatment without CSE exposure also enhanced ASL depth, indicative of the responsive nature of the MCC apparatus to augmented anion secretion, even in healthy non-CF monolayers (Figures 5A and 5B). In total, these data strongly indicate that the CFTR potentiator, ivacaftor, partially abrogates the downstream manifestations of acquired CFTR dysfunction induced by CSE on airway epithelia, ultimately resulting in increased airway surface hydration and ciliary beating necessary for efficient MCT. Collectively, these data demonstrate that CFTR potentiation represents an attractive therapeutic target to address mucus stasis in COPD, as demonstrated by models predictive of in vivo results in CF trials.

A number of mechanisms are known to contribute to acquired CFTR dysfunction; however, the mechanistic basis, relative importance, and impact on functional phenotype are still emerging. This article adds definitive details to this pathway, and in particular the importance and physiological significance of acute-acting mechanisms. In addition to biochemical changes in CFTR expression that occur on a chronic time frame, our results point out the importance of acute inhibition of CFTR activity through altered channel gating by CSE, and that its underlying molecular mechanism may be due to covalent modifications of the CFTR protein itself. Acute inhibition of ISC was immediate and robust, even in native epithelia, supporting previous observations of altered CFTR gating observed in CFTR-transduced Xenopus oocytes (22). Reversal by ivacaftor in the same studies lends credence to this observation, and rule out loss of channels as the primary cause (in addition to the nature of excised patch studies, where protein recycling machinery is absent). We further identified that purified CFTR undergoes chemical modification upon CSE exposure by Michael addition, modifying CFTR fragments with the acrolein moieties at key positions within the CFTR molecule. This provides key mechanistic information on the molecular level for how CFTR function is modified by smoking. It also is completely consistent with our prior observations that acrolein itself, a prominent component of cigarette smoke, confers abnormal gating and does so rapidly (7). CFTR has been shown to be susceptible to posttranslational modifications that alter its functional properties (16); hence, this is another example of the importance of posttranslational reactions modifying (or regulating) CFTR function.

The findings demonstrating the clear contribution of acute-acting pathways to acquired CFTR dysfunction do not discount the importance of chronic mechanisms that also clearly contribute. Reduced CFTR mRNA expression has been reported in both human airway cells exposed to cigarette smoke in vitro and nasal samples procured from human smokers (8, 11). Reduced CFTR mRNA transcripts have also been demonstrated in the lungs of patients with COPD at the time of explantation (32), although a decrease in CFTR mRNA expression was not observed in a recent study that relied on endobronchial sampling of airway basal cells (33). Protein levels are also affected by cigarette smoke, which induces CFTR internalization through a Ca2+-dependent pathway (4). This is consistent with reduced levels of surface CFTR expression that have been reported by our group (8); whether this is induced by Michael reactions that impact CFTR residues could be addressed in future studies.

Previous studies have demonstrated that cigarette smoking can contribute to acquired CFTR dysfunction, potentially contributing to the pathogenesis of airway disease, such as COPD, and in particular those with chronic bronchitis (48). However, most of these studies have evaluated the effects of chronic cigarette smoke exposure on ion transport or ASL depth at a single, chronic time point, and have not captured the kinetics of molecular mechanisms altered by smoke exposure. The functional anatomic imaging performed here clearly demonstrates the deleterious effects of cigarette smoke on ASL depth that occur within 60 minutes, providing strong evidence that cigarette smoke–induced acquired CFTR dysfunction contributes to the pathogenesis of COPD. Overall, the results support a number of epidemiologic studies that link chronic bronchitis symptoms and severity to the degree of CFTR abnormality (710).

Although evidence is unequivocal that smoking cessation is absolutely critical to limit disease progression, chronic bronchitis symptoms can often persist for a large number of patients with COPD who successfully quit smoking, and still others never successfully quit smoking despite the desire to do so; likewise, CFTR dysfunction also can persist despite smoking cessation, as shown by our group in two separate cohorts (7, 10). There are still large unmet therapeutic needs to be addressed, even in those individuals that have stopped smoking. Thus, reduced CFTR function in COPD-related chronic bronchitis represents an important therapeutic opportunity worthy of further exploration. Reversal of CFTR gating abnormalities induced by cigarette smoke, acrolein, hypoxia, or other factors is particularly attractive, as augmenting channel opening is the principal mechanism by which CFTR potentiators, such as ivacaftor, confers its benefit (13, 30). This does not discount the potential for other mechanisms, such as those that augment expression or address epigenetic regulation, which may also be of interest, especially when CFTR function is affected by other mechanisms, such as loss of cell differentiation and proteolytic degradation of the CFTR protein (34). Stimulation of a depleted number of residual channels, such as that observed in patients with R117H/5T CF who exhibit reduced CFTR activity due to a reduced number of channels, also may play a role (35). The recently published pilot evaluation of ivacaftor in patients with COPD confirms the feasibility of using CFTR modulators in COPD. Although this pilot trial was statistically underpowered and likely of too short in duration to detect definitive changes in lung function, there were improvements in CFTR activity and respiratory symptoms, especially in those patients whose CFTR activity was diminished at baseline (36).

Another important finding revealed by functional anatomic imaging is that the addition of ivacaftor, even when administered to healthy monolayers, increased ASL depth. This is consistent with clinical data showing that MCC in healthy individuals can be pharmacologically stimulated over basal rates (37), and is also compatible with the notion that CFTR is submaximally activated under healthy conditions and susceptible to stimulation when required, such as after the exposure to noxious stimuli or respiratory pathogens (38). This suggests the potential for CFTR potentiators or activators to offer a protective function even in those without genetic or acquired CFTR dysfunction, a concept worthy of prospective study.

The authors acknowledge the patients who allowed use of their cells and tissues to conduct these experiments, and Dr. Emily Falk Libby (The Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL) for assistance preparing the manuscript.

1. National Heart, Lung, and Blood Institute. Unpublished Tabulations of the National Health Interview Survey. 2010 [accessed 2016 Aug 20]. Available from: Data Source
2. Xu JQ, Kochanek KD, Murphy SL, Tejada-Vera B. Deaths: Final data for 2007. National vital statistics reports; vol 58 no 19. Hyattsville, MD: National Center for Health Statistics. 2010 [accessed 2016 Aug 20]. Available from:
3. National Heart, Lung, and Blood Institute. COPD: the more you know, the better for you and your loved ones. Sep 2013 [accessed 2014 Jun 10]. Available from:
4. Clunes LA, Davies CM, Coakley RD, Aleksandrov AA, Henderson AG, Zeman KL, Worthington EN, Gentzsch M, Kreda SM, Cholon D, et al. Cigarette smoke exposure induces CFTR internalization and insolubility, leading to airway surface liquid dehydration. FASEB J 2012;26:533545.
5. Kreindler JL, Jackson AD, Kemp PA, Bridges RJ, Danahay H. Inhibition of chloride secretion in human bronchial epithelial cells by cigarette smoke extract. Am J Physiol Lung Cell Mol Physiol 2005;288:L894L902.
6. Savitski AN, Mesaros C, Blair IA, Cohen NA, Kreindler JL. Secondhand smoke inhibits both Cl and K+ conductances in normal human bronchial epithelial cells. Respir Res 2009;10:120.
7. Raju SV, Jackson PL, Courville CA, McNicholas CM, Sloane PA, Sabbatini G, Tidwell S, Tang LP, Liu B, Fortenberry JA, et al. Cigarette smoke induces systemic defects in cystic fibrosis transmembrane conductance regulator function. Am J Respir Crit Care Med 2013;188:13211330.
8. Sloane PA, Shastry S, Wilhelm A, Courville C, Tang LP, Backer K, Levin E, Raju SV, Li Y, Mazur M, et al. A pharmacologic approach to acquired cystic fibrosis transmembrane conductance regulator dysfunction in smoking related lung disease. PLoS One 2012;7:e39809.
9. Dransfield MT, Wilhelm AM, Flanagan B, Courville C, Tidwell SL, Raju SV, Gaggar A, Steele C, Tang LP, Liu B, et al. Acquired cystic fibrosis transmembrane conductance regulator dysfunction in the lower airways in COPD. Chest 2013;144:498506.
10. Courville CA, Tidwell S, Liu B, Accurso FJ, Dransfield MT, Rowe SM. Acquired defects in CFTR-dependent β-adrenergic sweat secretion in chronic obstructive pulmonary disease. Respir Res 2014;15:25.
11. Cantin AM, Hanrahan JW, Bilodeau G, Ellis L, Dupuis A, Liao J, Zielenski J, Durie P. Cystic fibrosis transmembrane conductance regulator function is suppressed in cigarette smokers. Am J Respir Crit Care Med 2006;173:11391144.
12. Hassan F, Nuovo GJ, Crawford M, Boyaka PN, Kirkby S, Nana-Sinkam SP, Cormet-Boyaka E. MiR-101 and miR-144 regulate the expression of the CFTR chloride channel in the lung. PLoS One 2012;7:e50837.
13. Van Goor F, Hadida S, Grootenhuis PD, Burton B, Cao D, Neuberger T, Turnbull A, Singh A, Joubran J, Hazlewood A, et al. Rescue of CF airway epithelial cell function in vitro by a CFTR potentiator, VX-770. Proc Natl Acad Sci USA 2009;106:1882518830.
14. Rowe SM, Pyle LC, Jurkevante A, Varga K, Collawn J, Sloane PA, Woodworth B, Mazur M, Fulton J, Fan L, et al. DeltaF508 CFTR processing correction and activity in polarized airway and non-airway cell monolayers. Pulm Pharmacol Ther 2010;23:268278.
15. Jurkuvenaite A, Varga K, Nowotarski K, Kirk KL, Sorscher EJ, Li Y, Clancy JP, Bebok Z, Collawn JF. Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosis. J Biol Chem 2006;281:33293334.
16. McClure M, DeLucas LJ, Wilson L, Ray M, Rowe SM, Wu X, Dai Q, Hong JS, Sorscher EJ, Kappes JC, et al. Purification of CFTR for mass spectrometry analysis: identification of palmitoylation and other post-translational modifications. Protein Eng Des Sel 2012;25:714.
17. Liu L, Gardecki JA, Nadkarni SK, Toussaint JD, Yagi Y, Bouma BE, Tearney GJ. Imaging the subcellular structure of human coronary atherosclerosis using micro-optical coherence tomography. Nat Med 2011;17:10101014.
18. Liu L, Chu KK, Houser GH, Diephuis BJ, Li Y, Wilsterman EJ, Shastry S, Dierksen G, Birket SE, Mazur M, et al. Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography. PLoS One 2013;8:e54473.
19. van der Toorn M, Rezayat D, Kauffman HF, Bakker SJ, Gans RO, Koëter GH, Choi AM, van Oosterhout AJ, Slebos DJ. Lipid-soluble components in cigarette smoke induce mitochondrial production of reactive oxygen species in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol 2009;297:L109L114.
20. Lambert JA, Raju SV, Tang LP, McNicholas CM, Li Y, Courville CW, Faris RF, Coricor GE, Smoot LH, Mazur MM, et al. Cystic fibrosis transmembrane conductance regulator activation by roflumilast contributes to therapeutic benefit in chronic bronchitis. Am J Respir Cell Mol Biol 2014;50:549558.
21. Raju SV, Tate JH, Peacock SK, Fang P, Oster RA, Dransfield MT, Rowe SM. Impact of heterozygote CFTR mutations in COPD patients with chronic bronchitis. Respir Res 2014;15:18.
22. Moran AR, Norimatsu Y, Dawson DC, MacDonald KD. Aqueous cigarette smoke extract induces a voltage-dependent inhibition of CFTR expressed in Xenopus oocytes. Am J Physiol Lung Cell Mol Physiol 2014;306:L284L291.
23. Eiserich JP, van der Vliet A, Handelman GJ, Halliwell B, Cross CE. Dietary antioxidants and cigarette smoke–induced biomolecular damage: a complex interaction. Am J Clin Nutr 1995;62(6 suppl):1490S1500S.
24. Lambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM. Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-κB1 DNA binding domain. J Biol Chem 2007;282:1966619675.
25. Cai J, Bhatnagar A, Pierce WM Jr. Protein modification by acrolein: formation and stability of cysteine adducts. Chem Res Toxicol 2009;22:708716.
26. Uchida K, Kanematsu M, Morimitsu Y, Osawa T, Noguchi N, Niki E. Acrolein is a product of lipid peroxidation reaction: formation of free acrolein and its conjugate with lysine residues in oxidized low density lipoproteins. J Biol Chem 1998;273:1605816066.
27. Deshmukh HS, Case LM, Wesselkamper SC, Borchers MT, Martin LD, Shertzer HG, Nadel JA, Leikauf GD. Metalloproteinases mediate mucin 5AC expression by epidermal growth factor receptor activation. Am J Respir Crit Care Med 2005;171:305314.
28. Birket SE, Chu KK, Liu L, Houser GH, Diephuis BJ, Wilsterman EJ, Dierksen G, Mazur M, Shastry S, Li Y, et al. A functional anatomic defect of the cystic fibrosis airway. Am J Respir Crit Care Med 2014;190:421432.
29. Innes AL, Woodruff PG, Ferrando RE, Donnelly S, Dolganov GM, Lazarus SC, Fahy JV. Epithelial mucin stores are increased in the large airways of smokers with airflow obstruction. Chest 2006;130:11021108.
30. Jih KY, Hwang TC. Vx-770 potentiates CFTR function by promoting decoupling between the gating cycle and ATP hydrolysis cycle. Proc Natl Acad Sci USA 2013;110:44044409.
31. Flume PA, Liou TG, Borowitz DS, Li H, Yen K, Ordoñez CL, Geller DE; VX 08-770-104 Study Group. Ivacaftor in subjects with cystic fibrosis who are homozygous for the F508del-CFTR mutation. Chest 2012;142:718724.
32. Guimbellot JS, Fortenberry JA, Siegal GP, Moore B, Wen H, Venglarik C, Chen YF, Oparil S, Sorscher EJ, Hong JS. Role of oxygen availability in CFTR expression and function. Am J Respir Cell Mol Biol 2008;39:514521.
33. Tilley AE, Staudt MR, Salit J, Van de Graaf B, Strulovici-Barel Y, Kaner RJ, Vincent T, Agosto-Perez F, Mezey JG, Raby BA, et al. Cigarette smoking induces changes in airway epithelial expression of genes associated with monogenic lung disorders. Am J Respir Crit Care Med 2016;193:215217.
34. Le Gars M, Descamps D, Roussel D, Saussereau E, Guillot L, Ruffin M, Tabary O, Hong SS, Boulanger P, Paulais M, et al. Neutrophil elastase degrades cystic fibrosis transmembrane conductance regulator via calpains and disables channel function in vitro and in vivo. Am J Respir Crit Care Med 2013;187:170179.
35. Moss RB, Flume PA, Elborn JS, Cooke J, Rowe SM, McColley SA, Rubenstein RC, Higgins M; VX11-770-110 (KONDUCT) Study Group. Efficacy and safety of ivacaftor in patients with cystic fibrosis who have an Arg117His-CFTR mutation: a double-blind, randomised controlled trial. Lancet Respir Med 2015;3:524533.
36. Solomon GM, Raju SV, Dransfield MT, Rowe SM. Therapeutic approaches to acquired cystic fibrosis transmembrane conductance regulator dysfunction in chronic bronchitis. Ann Am Thorac Soc 2016;13:S169S176.
37. Bennett WD, Almond MA, Zeman KL, Johnson JG, Donohue JF. Effect of salmeterol on mucociliary and cough clearance in chronic bronchitis. Pulm Pharmacol Ther 2006;19:96100.
38. Schwarzer C, Wong S, Shi J, Matthes E, Illek B, Ianowski JP, Arant RJ, Isacoff E, Vais H, Foskett JK, et al. Pseudomonas aeruginosa homoserine lactone activates store-operated cAMP and cystic fibrosis transmembrane regulator–dependent Cl secretion by human airway epithelia. J Biol Chem 2010;285:3485034863.
Correspondence and requests for reprints should be addressed to Steven M. Rowe, M.D., M.S.P.H., 1918 University Boulevard, MCLM 706, Birmingham, AL 35294-0006. E-mail:

This work was supported by National Institutes of Health (NIH) grants 1R01 HL105487 and P30 DK072482 (S.M.R.), and Cystic Fibrosis Foundation grants R464-CF and CLANCY09Y0 (S.M.R.). S.V.R. is supported by American Lung Association grant RG-305752 and Flight Attendants Medical Research Association grant YFA130008. The mass spectrometer was purchased with funds from NIH Shared Instrumentation grant S10 RR027822 (S.B.).

Author Contributions: S.V.R. and S.M.R. conceived of the experiments. S.V.R., V.Y.L., L.L., C.M.M., S.K., P.A.S., L.T., P.L.J., W.W., L.W., K.J.M., and M.M. conducted the experiments; J.C.K., L.J.D., S.B., K.K., and G.J.T. provided reagents and techniques; S.V.R., C.M.M., S.K., L.W., and S.M.R. analyzed the data; S.M.R. wrote the manuscript; S.M.R. supervised the project; all authors approved of the manuscript submission and its contents.

Originally Published in Press as DOI: 10.1165/rcmb.2016-0226OC on September 1, 2016

Author disclosures are available with the text of this article at


No related items
Comments Post a Comment

New User Registration

Not Yet Registered?
Benefits of Registration Include:
 •  A Unique User Profile that will allow you to manage your current subscriptions (including online access)
 •  The ability to create favorites lists down to the article level
 •  The ability to customize email alerts to receive specific notifications about the topics you care most about and special offers
American Journal of Respiratory Cell and Molecular Biology

Click to see any corrections or updates and to confirm this is the authentic version of record