American Journal of Respiratory and Critical Care Medicine

Nontuberculous mycobacterial pulmonary disease (NTM PD) may be chronic, requiring lengthy and complex regimens over many months to years. After treatment, patients are often reinfected, with disease recurrence. NTM PD prevalence was estimated at 86,000 cases in the United States in 2010, surpassing pulmonary tuberculosis (TB), which was 9,272 in 2016 and declining; multiple studies in different U.S. and Canadian populations have indicated increasing prevalence since the mid-1990s (1). Increasing prevalence of NTM PD has also been documented in Australia, Asia, and Europe (2). Clinical care standards (3) would benefit from controlled clinical trials. To identify critical gaps in diagnosis, treatment, and prevention of NTM PD, the National Institute of Allergy and Infectious Diseases convened a workshop on September 26, 2017, bringing together diverse experts in mycobacterial disease. This report summarizes the key questions in host–pathogen interactions, molecular epidemiology and diagnostics, and vaccine/therapeutics. These key focus areas may help guide biomedical research efforts into this growing concern.

Host and pathogen factors are known to contribute to NTM PD. Certain body morphotypes and sex (4), structural lung abnormalities, genetic disorders affecting mucociliary clearance, and the use of immunosuppressive drugs, such as steroids and tumor necrosis factor (TNF)-α blockers, are associated with a higher risk of NTM PD (5). Impaired ciliary function predisposes to NTM disease (6). How airway clearance affects mycobacterial attachment and invasion must be characterized. Mycobacterium avium subsp. avium and Mycobacterium abscessus subsp. abscessus infection of human bronchial epithelial cells cultured at the air–liquid interface lead to down-regulation of cilia genes and up-regulation of cholesterol biosynthesis and proinflammatory markers, including IL-32 (7), suggesting significant immune signaling from respiratory epithelium per se. Further investigation into how contact between NTM and lung epithelia triggers immune responses, through the addition of immune cells to the air–liquid interface model, may identify key mechanisms that can be modulated. Distinct immune pathways may be relevant to NTM infection in cystic fibrosis (CF); the high prevalence of NTM PD among persons with CF (8) may facilitate studies of host factors that vary between those who do and do not have NTM PD. Interestingly, patients receiving TNF-α inhibitors (5) for inflammatory conditions have an elevated risk of NTM infection. Further research in all of these areas will help identify host biomarkers to identify disease activity.

Vaccines that are able to limit infection might have an ameliorative effect on NTM PD in susceptible hosts. Early studies in mice identified candidate antigens and approaches (9, 10). It is unclear whether shared antigens can elicit protection against the diverse NTM that cause disease (11). Reports of increased incidence of extrapulmonary NTM in Sweden and Finland after the end of bacillus Calmette-Guérin (BCG) vaccination to protect against TB suggest that BCG may provide collateral protection against extrapulmonary NTM (12, 13). However, no evidence is available regarding its effect on pulmonary NTM. Considering the different factors implicated in pulmonary versus disseminated NTM, a pulmonary vaccine, presumably inhaled, might be of interest to explore in NTM PD. Mycobacterial biofilms or microaggregates have been demonstrated in the lungs of patients with M. abscessus infections (14). M. avium subsp. hominissuis (15) biofilm appears to be important for invasion and infection (1518). Therefore, we need a better understanding of biofilms, including their structure, physiology, and how concomitant pathogens interact with each other. For example, some evidence exists that M. abscessus subsp. abscessus is able to degrade quinolone signals from Pseudomonas aeruginosa (19).

Biofilms may affect susceptibility to antibiotics, persistence, and antigen exposure, which in turn may affect immune responses and pathology. We need animal models that reflect critical characteristics of the pathogens in these unique niches to explore and vet promising drug candidates.

Significant genotypic and phenotypic diversity exists across and within NTM species. M. avium strains responsible for pulmonary infections may differ from those that lead to disseminated disease (20). The relative prevalence of NTM species varies with the underlying lung disease, such as CF or chronic obstructive pulmonary disease (COPD): for example, in Scotland, 68% of patients with CF had M. abscessus compared with 5% of patients without CF; in Japan, 24% of patients with M. abscessus had COPD versus 5% of patient with M. avium complex (MAC) (21, 22). A publicly accessible, well-characterized reference panel of NTM species is a critical first step in the systematic study of these organisms in a rigorous manner. However, considering the number of NTM species and strains that may be implicated in human disease, initial studies may best be focused on MAC and M. abscessus strains, because these are the most commonly encountered organisms in NTM PD (2, 3, 23), and M. abscessus strains are the most difficult to treat. In parallel, characterization of the species and distribution of NTM within and among patients may give insights into other species and strains that should be prioritized for research, as well as the mechanisms by which certain species or subspecies predominate in specific patient populations. A focused effort to understand pathogen diversity and distribution may identify common virulence characteristics as well as resistance phenotypes. Virulence factors have been identified for MAC (24), including those affecting biofilm formation, and virulence factors for M. abscessus have been studied in a number of cellular and animal models (25), Whole-genome sequencing may improve prediction of treatment response, but we must consider complex coinfections with different strains of an NTM species, multiple NTM species, and/or other bacteria and fungi. Within-patient diversity of NTM strains may be significant, and targeted deep-sequencing of patient samples may be necessary to understand population and strain diversity in various anatomical regions of the human lung. In vitro susceptibility and clinical response to macrolides and aminoglycosides are linked to genetic resistance markers for both M. abscessus and MAC that have the potential to serve as surrogates of phenotypic resistance (2628). Genome-wide association studies of mycobacteria with antimicrobial susceptibility testing in culture combined with SNP modeling may help further identify resistance conferring mutations.

Bacterial genetics could be characterized to help support molecular testing for diagnostics and possible prognostic precision. Currently, correlation of bacterial genetics and treatment outcomes in patients remains complex. However, before laboratory assays can be established that have the potential to aid in the development of prognostic tools, a more thorough understanding of the mechanisms of virulence in humans must be obtained.

Current laboratory procedures for mycobacteria were almost entirely developed around Mycobacterium tuberculosis. Improving recovery and identification of NTM is helped by a new selective mycobacterial medium (RGM) (29) and the increasing use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for mycobacterial identification. Additional simple-to-implement technologies may be required to leverage the diagnostic potential of markers of genetic and phenotypic variation in NTM species, determine antimicrobial susceptibility or resistance, and/or aid in the speciation of NTM in the clinical laboratory. Molecular methods may further contribute to subspecies characterization of NTM, such as a recently developed PCR-based technique for rapid identification of the presence or absence of a full-length erm(41) gene (30) in M. abscessus group, that could confer macrolide resistance, in addition to known drug resistance markers such as erm(41) position 28 C-T. More widespread access to future simpler point-of-care technologies will also facilitate rapid identification of NTM for improved timely treatment.

The current treatment recommendations for NTM PD vary by species and are frequently modified by treating physicians (3). On the basis of a limited number of trials and observational studies, culture conversion rates for the recommended MAC regimens are estimated at 65.7% (95% confidence interval, 53.3–77.4%) when drugs were taken for at least 1 year by patients who were macrolide susceptible and had previously untreated MAC (31). In contrast, M. abscessus infections and macrolide-resistant MAC are much more difficult to eradicate, and rates of culture conversion remain low (26, 32). Given the significant adverse events during NTM therapy, coupled with low efficacy against certain pathogens, better regimens are urgently needed.

Past drug screening efforts for M. tuberculosis found some compounds that were also active against MAC and M. abscessus (33). Drug discovery programs should screen separately for M. abscessus and MAC, because these organisms are phylogenetically distinct and compounds may be active against only one group. The genetic and phenotypic diversity within M. abscessus and MAC requires evaluation against a variety of strains or subspecies at an early step in the drug discovery pathway, as well as when existing antibacterial agents are evaluated for their efficacy against NTM (34).

Considering the various microenvironments that NTM occupy in the body, development of NTM-specific in vitro potency assays that mimic the state of the pathogen in the host are needed to better correlate in vitro susceptibility with clinical efficacy and speed identification of drug candidates (3). Models of slow- or nonreplicating “persister” populations of bacilli, and conditions reflecting intracellular growth, various levels of oxygen tension, nutrient starvation, as well as caseum and mucus, may be important. Pharmacokinetic in vitro assays that model “infection-site”–specific drug penetration (for instance penetration into caseum and mucus) have been developed for TB and may have utility for NTM. These assays should be paired with animal model studies that reflect human progressive pulmonary disease. Furthermore, multidrug therapy models are essential, as there is no credible monotherapy approach to NTM PD, all while keeping drug–drug interactions in mind; development efforts focused on regimens rather than single new chemicals would also be advantageous, as has recently been demonstrated for TB (35).

Clinical evaluation of novel drugs and regimens will require standardized case definitions, outcome measures, and comparator regimens, as well as the ability to conduct multicenter trials. Examples from TB (CDC Tuberculosis Trials Consortium) and HIV (NIH/National Institute of Allergy and Infectious Diseases AIDS Clinical Trial Group) clearly demonstrate the importance of clinical trial consortia for providing the necessary expertise, patient population, and data management infrastructure to conduct high-quality trials. Safety and efficacy endpoints, as well as required sample sizes to reach statistical significance, must be aligned with disease characteristics of the patient population to be enrolled. For example, endpoints based on microbiological culture conversion may only be feasible in patients with cavitary disease who present with significant bacterial burden in sputum. Although no consensus has been reached regarding ideal endpoints for efficacy trials in NTM PD, a recent publication did produce consensus definitions of microbiologic and functional endpoints (36), and a recent summary of patient research priorities has highlighted the importance of including quality-of-life outcomes as well (37).

Whole-genome sequencing has recently been used to characterize outbreaks of M. abscessus subsp. massiliense, showing evidence of possible person-to-person transmission (38). Although the prevalence and geographic extent of transmission remain controversial (3942), the need for further genetic and epidemiologic characterization of linked isolates is clear. Given the environmental hardiness of these organisms and the potential for fomite transmission (43), epidemiological investigations may provide insight into potential transmission routes in the presence and absence of direct patient contact. Environmental source investigations will require the development of validated processing protocols that are specific to M. abscessus and allow sampling of diverse potential environmental reservoirs for this species. Improved recovery of M. abscessus isolates will aid outbreak investigations, allow the characterization of the genetic diversity of environmental M. abscessus strains, and address whether the globally distributed “clustered” strains of M. abscessus are also present in the environment.

Polyclonal infection is likely common, as is reinfection after treatment for MAC and M. abscessus (44, 45). Development of biomarkers that differentiate initial, persistent, and subsequent infections will necessitate the establishment of longitudinal patient cohorts to collect well-characterized clinical and microbial specimens and obtain well-curated genetic data. Deep sequencing of microbial genomes from patient samples will also contribute to the characterization of the dynamics of simultaneous and sequential infections with mixed NTM strains and other intercurrent infections.

The complexity and diversity of NTM PD, and the difficulty of modeling it in animals, will require new tools. It seems prudent to focus on priority pathogens, for instance M. abscessus and MAC. Animal studies may allow the development of initial hypotheses. The analysis of patient databases to conduct genetic and clinical research will facilitate the establishment of best practices that can subsequently be extended to the study of other NTM infections. The “Collaborative Cross” panel of mouse strains reflects extensive host genetic heterogeneity and has promise to advance the field but will have to be directed toward NTM pathogens to be useful (46). Animal models of airway defects similar to those in humans are essential (25). The recent nonhuman primate model may prove useful (47). Bioinformatic tools and patient databases will be required to facilitate international comparison of data. Publicly accessible genetic data from human studies often lack linked clinical metadata, and although CF-specific databases for patients with NTM exist in both the United States (48) and the United Kingdom (49), as do COPD (50) and bronchiectasis (51) registries, the amount of NTM-specific information captured is variable and is lacking linked genetic data. Harmonization of sequencing approaches and bioinformatic analyses, as well as development of standardized clinical protocols with common data elements and case report forms, will maximize the value of patient data and infrastructure.

Because of its increasing prevalence, difficult diagnosis and treatment, and extremely high recurrence, increased research is needed for NTM PD. MAC and M. abscessus require more pathogenesis research and drug discovery. Standardized protocols for whole-genome sequencing analysis and clinical data collection are needed to leverage collaborative research opportunities. Development of vaccines, drugs, and diagnostics will need to be specifically tailored to the hosts and pathogens, as one size will not fit all.

1. Daniel-Wayman S, Adjemian J, Prevots DR. Epidemiology of nontuberculous mycobacteria in the United States. In: Griffith DE, editor. Nontuberculous mycobacterial disease: a comprehensive approach to diagnosis and management. Springer; 2018. pp 145162.
2. Prevots DR, Marras TK. Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med 2015;36:1334.
3. Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, et al.; ATS Mycobacterial Diseases Subcommittee; American Thoracic Society; Infectious Disease Society of America. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007;175:367416. [Published erratum appears in Am J Respir Crit Care Med 175:744–745.]
4. Kim RD, Greenberg DE, Ehrmantraut ME, Guide SV, Ding L, Shea Y, et al. Pulmonary nontuberculous mycobacterial disease: prospective study of a distinct preexisting syndrome. Am J Respir Crit Care Med 2008;178:10661074.
5. Brode SK, Jamieson FB, Ng R, Campitelli MA, Kwong JC, Paterson JM, et al. Increased risk of mycobacterial infections associated with anti-rheumatic medications. Thorax 2015;70:677682.
6. Fowler CJ, Olivier KN, Leung JM, Smith CC, Huth AG, Root H, et al. Abnormal nasal nitric oxide production, ciliary beat frequency, and Toll-like receptor response in pulmonary nontuberculous mycobacterial disease epithelium. Am J Respir Crit Care Med 2013;187:13741381.
7. Matsuyama M, Martins AJ, Shallom S, Kamenyeva O, Kashyap A, Sampaio EP, et al. Transcriptional response of respiratory epithelium to nontuberculous mycobacteria. Am J Respir Cell Mol Biol 2018;58:241252.
8. Adjemian J, Olivier KN, Prevots DR. Nontuberculous mycobacteria among patients with cystic fibrosis in the United States: screening practices and environmental risk. Am J Respir Crit Care Med 2014;190:581586.
9. Le Moigne V, Rottman M, Goulard C, Barteau B, Poncin I, Soismier N, et al. Bacterial phospholipases C as vaccine candidate antigens against cystic fibrosis respiratory pathogens: the Mycobacterium abscessus model. Vaccine 2015;33:21182124.
10. Le Moigne V, Belon C, Goulard C, Accard G, Bernut A, Pitard B, et al. MgtC as a host-induced factor and vaccine candidate against Mycobacterium abscessus infection. Infect Immun 2016;84:28952903.
11. Kaufmann SH, Lange C, Rao M, Balaji KN, Lotze M, Schito M, et al. Progress in tuberculosis vaccine development and host-directed therapies—a state of the art review. Lancet Respir Med 2014;2:301320.
12. Romanus V, Hallander HO, Wåhlén P, Olinder-Nielsen AM, Magnusson PHW, Juhlin I. Atypical mycobacteria in extrapulmonary disease among children: incidence in Sweden from 1969 to 1990, related to changing BCG-vaccination coverage. Tuber Lung Dis 1995;76:300310.
13. Kontturi A, Soini H, Ollgren J, Salo E. Increase in childhood nontuberculous mycobacterial infections after bacille Calmette-Guérin coverage drop: a nationwide, population-based retrospective study, Finland, 1995-2016. Clin Infect Dis 2018;67:12561261.
14. Fennelly KP, Ojano-Dirain C, Yang Q, Liu L, Lu L, Progulske-Fox A, et al. Biofilm formation by Mycobacterium abscessus in a lung cavity. Am J Respir Crit Care Med 2016;193:692693.
15. Babrak L, Danelishvili L, Rose SJ, Kornberg T, Bermudez LE. The environment of “Mycobacterium avium subsp. hominissuis” microaggregates induces synthesis of small proteins associated with efficient infection of respiratory epithelial cells. Infect Immun 2015;83:625636.
16. Yamazaki Y, Danelishvili L, Wu M, Hidaka E, Katsuyama T, Stang B, et al. The ability to form biofilm influences Mycobacterium avium invasion and translocation of bronchial epithelial cells. Cell Microbiol 2006;8:806814.
17. Halloum I, Carrère-Kremer S, Blaise M, Viljoen A, Bernut A, Le Moigne V, et al. Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent. Proc Natl Acad Sci USA 2016;113:E4228E4237.
18. Rose SJ, Bermudez LE. Mycobacterium avium biofilm attenuates mononuclear phagocyte function by triggering hyperstimulation and apoptosis during early infection. Infect Immun 2014;82:405412.
19. Birmes FS, Wolf T, Kohl TA, Rüger K, Bange F, Kalinowski J, et al. Mycobacterium abscessus subsp. abscessus is capable of degrading Pseudomonas aeruginosa quinolone signals. Front Microbiol 2017;8:339.
20. Uchiya K, Takahashi H, Yagi T, Moriyama M, Inagaki T, Ichikawa K, et al. Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease. PLoS One 2013;8:e71831.
21. Nagano H, Kinjo T, Nei Y, Yamashiro S, Fujita J, Kishaba T. Causative species of nontuberculous mycobacterial lung disease and comparative investigation on clinical features of Mycobacterium abscessus complex disease: a retrospective analysis for two major hospitals in a subtropical region of Japan. PLoS One 2017;12:e0186826.
22. Russell CD, Claxton P, Doig C, Seagar AL, Rayner A, Laurenson IF. Non-tuberculous mycobacteria: a retrospective review of Scottish isolates from 2000 to 2010. Thorax 2014;69:593595.
23. Spaulding AB, Lai YL, Zelazny AM, Olivier KN, Kadri SS, Prevots DR, et al. Geographic distribution of nontuberculous mycobacterial species identified among clinical isolates in the United States, 2009-2013. Ann Am Thorac Soc 2017;14:16551661.
24. Jeffrey B, Rose SJ, Gilbert K, Lewis M, Bermudez LE. Comparative analysis of the genomes of clinical isolates of Mycobacterium avium subsp. hominissuis regarding virulence-related genes. J Med Microbiol 2017;66:10631075.
25. Bernut A, Herrmann JL, Ordway D, Kremer L. The diverse cellular and animal models to decipher the physiopathological traits of Mycobacterium abscessus infection. Front Cell Infect Microbiol 2017;7:100.
26. Griffith DE, Brown-Elliott BA, Langsjoen B, Zhang Y, Pan X, Girard W, et al. Clinical and molecular analysis of macrolide resistance in Mycobacterium avium complex lung disease. Am J Respir Crit Care Med 2006;174:928934.
27. Mougari F, Bouziane F, Crockett F, Nessar R, Chau F, Veziris N, et al. Selection of resistance to clarithromycin in Mycobacterium abscessus subspecies. Antimicrob Agents Chemother 2016;61:e00943-16.
28. Brown-Elliott BA, Iakhiaeva E, Griffith DE, Woods GL, Stout JE, Wolfe CR, et al. In vitro activity of amikacin against isolates of Mycobacterium avium complex with proposed MIC breakpoints and finding of a 16S rRNA gene mutation in treated isolates. J Clin Microbiol 2013;51:33893394.
29. Plongla R, Preece CL, Perry JD, Gilligan PH. Evaluation of RGM medium for isolation of nontuberculous mycobacteria from respiratory samples from patients with cystic fibrosis in the United States. J Clin Microbiol 2017;55:14691477.
30. Shallom SJ, Moura NS, Olivier KN, Sampaio EP, Holland SM, Zelazny AM. New real-time PCR assays for detection of inducible and acquired clarithromycin resistance in the Mycobacterium abscessus group. J Clin Microbiol 2015;53:34303437.
31. Diel R, Nienhaus A, Ringshausen FC, Richter E, Welte T, Rabe KF, et al. Microbiologic outcome of interventions against Mycobacterium avium complex pulmonary disease: a systematic review. Chest 2018;153:888921.
32. Jarand J, Levin A, Zhang L, Huitt G, Mitchell JD, Daley CL. Clinical and microbiologic outcomes in patients receiving treatment for Mycobacterium abscessus pulmonary disease. Clin Infect Dis 2011;52:565571.
33. Low JL, Wu ML, Aziz DB, Laleu B, Dick T. Screening of TB actives for activity against nontuberculous mycobacteria delivers high hit rates. Front Microbiol 2017;8:1539.
34. Aziz DB, Low JL, Wu ML, Gengenbacher M, Teo JWP, Dartois V, et al. Rifabutin is active against Mycobacterium abscessus complex. Antimicrob Agents Chemother 2017;61:e00155-17.
35. Murray S, Mendel C, Spigelman M. TB Alliance regimen development for multidrug-resistant tuberculosis. Int J Tuberc Lung Dis 2016;20:3841.
36. van Ingen J, Aksamit T, Andrejak C, Bottger EC, Cambau E, Daley C, et al.; NTM-NET. Treatment outcome definitions in nontuberculous mycobacterial pulmonary disease: an NTM-NET consensus statement. Eur Respir J 2018;51:1800170.
37. Henkle E, Aksamit T, Barker A, Daley CL, Griffith D, Leitman P, et al.; NTMRC Patient Advisory Panel. Patient-centered research priorities for pulmonary nontuberculous Mycobacteria (NTM) infection: an NTM Research Consortium workshop report. Ann Am Thorac Soc 2016;13:S379S384.
38. Bryant JM, Grogono DM, Greaves D, Foweraker J, Roddick I, Inns T, et al. Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. Lancet 2013;381:15511560.
39. Tettelin H, Davidson RM, Agrawal S, Aitken ML, Shallom S, Hasan NA, et al. High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. Emerg Infect Dis 2014;20:364371.
40. Davidson RM, Hasan NA, de Moura VC, Duarte RS, Jackson M, Strong M. Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains. Infect Genet Evol 2013;20:292297.
41. Harris KA, Underwood A, Kenna DT, Brooks A, Kavaliunaite E, Kapatai G, et al. Whole-genome sequencing and epidemiological analysis do not provide evidence for cross-transmission of Mycobacterium abscessus in a cohort of pediatric cystic fibrosis patients. Clin Infect Dis 2015;60:10071016.
42. Bryant JM, Grogono DM, Rodriguez-Rincon D, Everall I, Brown KP, Moreno P, et al. Emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium. Science 2016;354:751–757.
43. Malcolm KC, Caceres SM, Honda JR, Davidson RM, Epperson LE, Strong M, et al. Mycobacterium abscessus displays fitness for fomite transmission. Appl Environ Microbiol 2017;83:e00562-17.
44. Wallace RJ Jr, Brown-Elliott BA, McNulty S, Philley JV, Killingley J, Wilson RW, et al. Macrolide/azalide therapy for nodular/bronchiectatic Mycobacterium avium complex lung disease. Chest 2014;146:276282.
45. Lee BY, Kim S, Hong Y, Lee SD, Kim WS, Kim DS, et al. Risk factors for recurrence after successful treatment of Mycobacterium avium complex lung disease. Antimicrob Agents Chemother 2015;59:29722977.
46. Smith CM, Proulx MK, Olive AJ, Laddy D, Mishra BB, Moss C, et al. Tuberculosis susceptibility and vaccine protection are independently controlled by host genotype. MBio 2016;7:e01516-16.
47. Winthrop K, Rivera A, Engelmann F, Rose S, Lewis A, Ku J, et al. A rhesus macaque model of pulmonary nontuberculous mycobacterial disease. Am J Respir Cell Mol Biol 2016;54:170176.
48. Cystic Fibrosis Foundation. Patient registry. 2017 [accessed 2019 Mar 13]. Available from: https://www.cff.org/Research/Researcher-Resources/Patient-Registry/.
49. Cystic Fibrosis Trust. UK Cystic Fibrosis registry. 2018 [accessed 2019 Mar 13]. Available from: https://www.cysticfibrosis.org.uk/the-work-we-do/uk-cf-registry.
50. The COPD Foundation. COPD patient powered research network: for researchers. The COPD Foundation; 2018 [accessed 2019 Mar 13]. Available from: https://www.copdfoundation.org/Research/COPD-Patient-Powered-Research-Network/For-Researchers.aspx.
51. The COPD Foundation. Bronchiectasis and NTM research registry. The COPD Foundation; 2018 [accessed 2019 Mar 13]. Available from: https://www.copdfoundation.org/Research/Bronchiectasis-Research-Registry/Learn-More.aspx.
Correspondence and requests for reprints should be addressed to D. Rebecca Prevots, Ph.D., M.P.H., National Institute of Allergy and Infectious Diseases, NIH, 8 West Drive, MSC 2665, Bethesda, MD 20892. E-mail: .

Supported by the intramural and extramural (Division of Microbiology and Infectious Diseases) programs of the National Institute of Allergy and Infectious Diseases, with contributions by the NHLBI, both of the NIH.

Originally Published in Press as DOI: 10.1164/rccm.201807-1273PP on November 14, 2018

Author disclosures are available with the text of this article at www.atsjournals.org.

Related

No related items
Comments Post a Comment




New User Registration

Not Yet Registered?
Benefits of Registration Include:
 •  A Unique User Profile that will allow you to manage your current subscriptions (including online access)
 •  The ability to create favorites lists down to the article level
 •  The ability to customize email alerts to receive specific notifications about the topics you care most about and special offers
American Journal of Respiratory and Critical Care Medicine
199
8

Click to see any corrections or updates and to confirm this is the authentic version of record