Rationale: Pulmonary nontuberculous mycobacterial (PNTM) disease has increased over the past several decades, especially in older women. Despite extensive investigation, no consistent immunological abnormalities have been found. Using evidence from diseases such as cystic fibrosis and primary ciliary dyskinesia, in which mucociliary dysfunction predisposes subjects to high rates of nontuberculous mycobacterial disease that increase with age, we investigated correlates of mucociliary function in subjects with PNTM infections and healthy control subjects.
Objectives: To define ex vivo characteristics of PNTM disease.
Methods: From 2009 to 2012, 58 subjects with PNTM infections and 40 control subjects were recruited. Nasal nitric oxide (nNO) was determined at the time of respiratory epithelial collection. Ciliary beat frequency at rest and in response to Toll-like receptor (TLR) and other agonists was determined using high-speed video microscopy.
Measurements and Main Results: We found decreased nNO production, abnormally low resting ciliary beat frequency, and abnormal responses to agonists of TLR2, -3, -5, -7/8, and -9 in subjects with PNTM compared with healthy control subjects. The low ciliary beat frequency in subjects with PNTM was normalized ex vivo by augmentation of the NO–cyclic guanosine monophosphate pathway without normalization of their TLR agonist responses.
Conclusions: Impaired nNO, ciliary beat frequency, and TLR responses in PNTM disease epithelium identify possible underlying susceptibility mechanisms as well as possible avenues for directed investigation and therapy.
Pulmonary nontuberculous mycobacterial disease has increased over the past several decades, especially in older women. Despite a distinctive endomorphotype, no consistent immunological abnormalities have been found.
This study describes functional cellular characteristics that distinguish subjects with pulmonary nontuberculous mycobacterial disease from control subjects that are suggestive of a genetic predisposition. These differences also provide a possible therapeutic intervention.
Nontuberculous mycobacteria (NTM) are widely distributed in the environment but rarely cause disease (1). Disseminated disease occurs only in the setting of relatively rare specific host immune defects affecting IFN-γ or IL-12 activity (2–4). Pulmonary NTM (PNTM) disease occurs in conditions with underlying structural lung damage or impaired mucociliary clearance, such as cystic fibrosis (CF) or primary ciliary dyskinesia (PCD). The rate of PNTM in the general population has been increasing over the past few decades, especially in elderly women (5–8).
PNTM infection in postmenopausal women without recognized preexisting conditions was described in 1989 (9). The clinical phenotype includes lean and tall women whose onset of symptoms occurs in the sixth decade (10–14). These women also have high rates of scoliosis (51%), pectus excavatum (11%), mitral valve prolapse (9%), and mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene (36%) (15). The likely role of genetic factor(s) contributing to PNTM susceptibility is evidenced by a case-control study that favors a “susceptible persons” model of PNTM disease (16, 17) and familial clustering of PNTM disease (18, 19). Despite the importance of immune control for disseminated NTM disease, patients with PNTM have relatively normal lymphocyte subsets and intact IL-12/IFN -γ axes (15, 20–22).
Mucociliary clearance is a critical determinant of PNTM infections, as evinced by the elevated rates of disease in CF and PCD (23). In both of these groups, immune function appears to be relatively normal despite the fact that the rate of PNTM is high and increases dramatically with age (24, 25). Therefore, considering the importance of respiratory epithelial cells in mucociliary clearance and their impairment in CF and PCD, we hypothesized that respiratory epithelium might be critical to PNTM disease predisposition. Mucociliary function reflects the composite and dynamic interaction of ciliary beat frequency (CBF), ciliary waveform, and mucus viscosity. Mucus clearance is linearly dependent on CBF (26, 27), and nitric oxide (NO) (28) is known to be involved in regulating CBF. NO is released from the sinus respiratory epithelium and is thought to affect tissue repair and pulmonary arterial pressures and has a direct toxic effect on certain pathogens (29).
We found reductions in nasal NO (nNO) production and CBF in patients with PNTM. Patients with PNTM also had decreased CBF responses to selected Toll-like receptor (TLR) agonists. Most importantly, PNTM patient epithelial cell CBF could be normalized ex vivo by therapeutic augmentation of the NO–cyclic guanosine monophosphate (cGMP) pathway. These data provide a mechanistic basis for susceptibility to PNTM disease that can be targeted. Some preliminary results from this study were previously reported in the form of conference abstracts (30).
A detailed description of the methods is provided in the online supplement.
Recruitment of patients with PNTM, control subjects with disease, and healthy control subjects occurred from November 2009 to August 2012 at the Clinical Center, National Institutes of Health, Bethesda, Maryland. All patients and normal volunteers provided informed consent under approved National Institutes of Health protocols. All patients with PNTM had microbiologic and radiographic evidence of PNTM infection and fulfilled the American Thoracic Society criteria for PNTM disease (10, 15, 18). Patients with PNTM did not meet diagnostic criteria for CF or PCD. Control patients with CF had abnormal sweat chloride tests and known disease-causing mutations in CFTR. Patients diagnosed with PCD met published criteria (24, 31). CFTR status was determined by full gene sequence of CFTR (Ambry Genetics, Aliso Viejo, CA).
Primary human respiratory epithelial cells were collected as described in the online supplement and as previously described (24). Primary patient epithelial samples and normal human bronchial epithelial (NHBE) cells (Lonza, Walkersville, MD) were visualized at time of collection (day of collection [DOC]) and after mucociliary differentiation at the air–liquid interface (ALI).
Symptoms of acute upper respiratory tract infection at the time of collection were associated with decreased CBF when compared with the individual’s baseline (data not shown). We included only CBF measurements taken in the absence of symptomatic upper respiratory tract infection and without evidence of inflammation by visual inspection.
nNO was measured by direct sampling through a NO analyzer (model 280i; Sievers Instrument, Boulder, CO) and reported as steady-state production of NO in nl/min (24).
TLR location and reactivity differ between lung epithelium and leukocytes (32). Stimulating TLRs in airway epithelial cells requires higher agonist concentrations than for phagocytic cells (33). Samples were stimulated with single doses of TLR agonists (Invivogen, San Diego, CA): TLR2, 10 μg/ml Pam3CSK4; TLR3, 25 μg/ml poly(I:C) high molecular weight; TLR4, 5 μg/ml ultrapure Escherichia coli K12–derived LPS; TLR5, 100 ng/ml recombinant Flagellin; TLR7/8, 5 μg/ml imidazoquinoline compound R848 derivative CL097; TLR9, 4 μg/ml ODN 2006 Type B CpG oligonucleotide. Samples were also stimulated with 1 mM 8-bromoguanosine 3′,5′-cyclic monophosphate sodium salt (8-Br-cGMP) (Sigma, St. Louis, MO), sildenafil citrate (10 μM) (Tocris, Bristol, UK), or 1 mM S-nitroso-N-acetyl-d,l-penicillamine (SNAP) (Sigma). Clinical strains of Mycobacterium avium (MAC) and Mycobacterium abscessus (MAB) were cultured as previously described (34). Respiratory epithelial infection experiments were done in antibiotic/antifungal-free ALI media.
Supernatants from select healthy control and PNTM ALI cultures were examined for IL-6, IL-8, TNF-α, G-CSF, IFN-γ–inducible protein 10, and monocyte chemoattractant protein-1 in duplicate using bioluminescent beads (Bio-Rad, Hercules, CA) as previously described (15).
Data, including figures, are expressed as mean ± SD. Statistical testing was done using two-tailed Student’s t test with Welch’s correction and one-way ANOVA with Bonferroni correction.
Fifty-eight patients with PNTM, eight patients with PCD, five patients with CF, one IRAK4-deficient patient (35), and 41 healthy control subjects seen at the National Institutes of Health from 2009 to 2012 were recruited. The PNTM cohort has been previously described (15) (Tables 1 and 2 and see the online supplement).
Characteristics | Patients with PNTM (n = 58) | PCD (n = 8) | CF (n = 5) | Healthy Control Subjects (n = 41) |
---|---|---|---|---|
Mean ± SD age, yr | 63 ± 9 | 36 ± 18 | 46 ± 14 | 40 ± 15 |
Female gender | 51 (88)* | 7 (88) | 2 (40) | 36 (88) |
Ethnicity | ||||
White | 54 (93) | 5 (62) | 5 (100) | 26 (63) |
Asian | 4 (7) | 1 (13) | 0 (0) | 3 (7) |
Never smokers | 33 (57) | 8 (100) | 5 (100) | nd |
CFTR carriers | 14 (24) | 3 (38) | 5 (100) | nd |
Scoliosis | 30 (52) | nd | nd | nd |
Joint hypermobility | 12 (21) | nd | nd | nd |
Positive thumb–wrist sign | 12 (21) | nd | nd | nd |
Mitral valve prolapse | 4 (7) | 0 (0) | nd | nd |
Cough | 55 (95) | 8 (100) | 5 (100) | nd |
Bronchiectasis | 58 (100) | 8 (100) | 5 (100) | nd |
NTM positive sputum at collection | 31 (53) | 5 (63) | 3 (60) | nd |
On NTM therapy at collection | 31 (53) | 6 (75) | 5 (100) | nd |
Elevated inflammatory markers at collection | 39 (67) | 6 (75) | 2 (40) | nd |
Macrolide exposure at collection | 24 (47) | 4 (50) | 5 (100) | nd |
Immunomodulatory drugs | 4 (7) | 0 (0) | 2 (40) | nd |
BMI ± SD | 21.7 ± 4.7 | 22.3 ± 8.3 | 26.3 ± 6.5 | nd |
Mycobacteria | ||||
MAC | 41 (71) | 3 (60) | 1 (33) | |
MAB | 6 (10) | 2 (40) | 2 (67) | |
MAC and MAB | 10 (17) | |||
Mycobacterium xenopi | 1 (2) |
Infection History | Patients with PNTM (n = 58) | PCD (n = 8) | CF (n = 5) |
---|---|---|---|
Bacteria | P. aeruginosa, n = 18 | P. aeruginosa, n = 6 | P. aeruginosa, n = 2 |
S. maltophilia, n = 6 | S. pneumonia, n = 2 | Staphylococcus species, n = 2 | |
Achromobacter species, n = 4 | Staphylococcus aureus, n = 1 | Burkholderia gladioli, n = 2 | |
Other bacteria, n = 12 | Other bacteria, n = 2 | ||
Fungi | Aspergillus species, n = 25 | Penicillium species, n = 3 | Aspergillus species, n = 3 |
Penicillium species, n = 18 | Aspergillus species, n = 2 | Penicillium species, n = 3 | |
Dactylaria gallopava, n = 5 | Fusarium species n = 2 | Fusarium species n = 1 | |
Other fungi, n = 90 | Other fungi, n = 19 | Other fungi, n = 5 |
To determine CBF in PNTM patient airway epithelium, we examined cells on DOC (Figure 1a). Healthy control subjects (n = 41) had a mean CBF of 10.03 ± 0.97 Hz, comparable to published values (36). Patients with PCD (n = 8) were studied as a control group known to have decreased CBF (5.0 ± 5.5 Hz, similar to published reports [37]). Patients with CF (n = 5; CBF, 10.8 ± 1.1 Hz) were included as a control population that has chronic infections. Patients with PNTM had a CBF of 7.97 ± 1.09 Hz, significantly different from healthy subjects, patients with PCD, and control subjects with CF. CF CBF was similar to healthy control CBF as previously reported (38).
The differences between healthy control and PNTM CBF were present when measured at room temperature (22°C) and at physiological temperature (37°C) (see Figure E1a in the online supplement) (39). Similar to prior reports (40), CBF did not vary greatly by age in healthy control subjects or in patients with PNTM (Figure 1b). CBF on DOC cells was repeatedly measured in one subject throughout the study and did not significantly change (Figure E1b). To control for confounding factors not related to ciliary function (e.g., host inflammatory mediators) that might influence DOC samples, we differentiated a subset of healthy control and PNTM patient cells at the ALI (Figure 1c). In contrast, in subjects with chronic sinusitis the depressed CBF is transient and is suspected to be due to host inflammatory response (41) because the decreased CBF quickly normalizes when the samples are cultured ex vivo.
Because cilia length could affect ciliary function (42), we examined it in our patient population. Cilia length was the same for healthy control subjects (6.08 ± 0.85 μm) and patients with PNTM (6.14 ± 1.04 μm) (Figure E1c).
There is a high prevalence of CFTR mutations in patients with PNTM. We examined whether the presence of a CFTR mutation correlated with CBF and found that patients with PNTM had lower CBF regardless of CFTR mutation status (Figure 1d). Therefore, CFTR mutations do not seem to cause reduced CBF in patients with PNTM. No other PNTM characteristic segregated with CBF.
Because patients with PNTM harbor mycobacteria, bacterial growth per se could account for depression of PNTM patient CBF. To test if NTM infection played a role in the cultures, PNTM and healthy control ALI cultures were tested for NTM infection. No organisms were recovered after 6 weeks of culture (data not shown). To further assess whether mycobacterial infection could inhibit CBF, NHBE cells were cultured at the ALI and exposed to MAB or MAC every 3 days over an 8-day course (multiplicity of infection, 5:1). A significant increase in CBF was observed in NHBE cultures exposed to MAB or MAC compared with uninfected cultures (Figure 1e). The increase in CBF was similar in MAB- and MAC-infected cultures. These data suggest that infection does not account for the reduction in CBF seen in patients with PNTM.
Soluble factors can modify ciliary motility (43). To determine if PNTM epithelial cells were producing soluble factors that reduced CBF, we transferred conditioned media from PNTM or NHBE cultures onto uninfected NHBE cultures over the course of 2 weeks. PNTM-conditioned media did not alter CBF in NHBE cultures, suggesting that a soluble factor from PNTM epithelial cells is not responsible for the decreased CBF seen in PNTM patient cells (Figure 1f).
Although conditioned media from unstimulated PNTM epithelial cells did not induce lowered CBF in NHBE cultures, we wondered whether differential induction of soluble factors after stimulation could explain the altered CBF. To assess this, we used TLR agonists to stimulate respiratory epithelial cells differentiated at ALI from healthy volunteers and patients with PNTM. No significant differences were noted between patients and healthy control subjects for IL-6, IL-8, TNF-α, IFN-γ–inducible protein 10, G-CSF, and monocyte chemoattractant protein-1 responses (data not shown). Epithelial cell cytokine responses to TLR agonist stimulation were also not altered in patients with PNTM.
We also examined the CBF response to TLR agonists. TLR agonists differentially regulate respiratory epithelial CBF. In healthy control subjects, the bacterial TLR2 (Pam3CSK4), TLR4 (LPS), TLR5 (Flagellin), and TLR9 (CpG) agonists increase absolute CBF, whereas agonists to viral TLRs, TLR3 [poly(I:C)], and TLR7/8 (CL097) led to decreased absolute CBF (Figure 2a). Patients with PNTM, in contrast, had a depressed response to TLR4 (LPS), TLR9 (CpG), TLR2 (Pam3CSK4), and TLR5 (Flagellin) agonists but still had a significant increase in absolute CBF (Figure 2b). Viral TLR agonists TLR3 [poly(I:C)] and TLR7/8 (CL097) did not affect CBF in patients with PNTM. The absence of response to viral TLR agonist is significant because those agonists decrease CBF in healthy control subjects.
To explore the role of innate immune signaling, the respiratory epithelial cells of an IRAK4-deficient patient (35) were examined. The patient does not have a depressed baseline CBF, and the CBF response to TLR agonists was as expected because the only significant response was to the TLR3 [poly(I:C)] agonist (Figure 2c).
The absolute CBF values, baseline and in response to TLR agonists, are important; it is also informative to consider the percent change from baseline CBF induced by TLR agonists. Patients with PNTM are less responsive to all TLR agonists tested except for TLR4 (LPS) (Figure 2d). PNTM respiratory epithelial cells are more responsive to TLR4 (LPS) agonist than cells from healthy control subjects. Similar CBF responses to TLR agonists were seen in ALI cultures (data not shown).
To examine whether mycobacterial infection influenced the TLR response profile of epithelial cells, NHBE ALI cultures were infected with two rounds of MAB or MAC (multiplicity of infection, 5:1) for 8 days and then challenged with TLR agonists (Figure 2e; Figures E1d and E1e). Infection with either mycobacterial strain induced a TLR response profile that was not observed in uninfected NHBE cultures or in PNTM cells (Figure 2e). When infected NHBE ALI cultures were stimulated with TLR agonists, a further increase in CBF was observed with all TLR agonists (Figures E1d and E1e). This was most dramatic with the viral TLR (TLR3 and TLR7/8) agonists.
nNO has been shown to be positively correlated with CBF and to influence CBF (44). We found that healthy individuals had a mean nNO of 316 ± 76 nl/min, similar to prior reports (45). Patients with known PCD who are known to have dysregulated NO had mean nNO levels of 26 ± 24 nl/min. Patients with PNTM had mean nNO levels of 237 ± 77 nl/min, significantly different from healthy and PCD control subjects (Figure 3a). As previously reported, intercohort CBF and exhaled nNO production were positively correlated for all patients (36) (Figure 3b).
Decreased nNO at the population level correlated with reduced baseline CBF in patients with PNTM. This correlation warranted further exploration because estrogen is involved in the regulation of the NO–cGMP pathway. Therefore, DOC cells from healthy control subjects and patients with PNTM were exposed to 8Br-cGMP, a cGMP analog that increases CBF through the NO–cGMP pathway (46). Stimulation led to significant increase in CBF in healthy control subjects and patients with PNTM. A significant increase above preexposure baseline CBF continued to be observed after 8 hours in patients with PNTM but not in healthy control subjects (Figure 4a). Increases in CBF were similar for healthy control subjects and patients with PNTM across a broad dose range (Figure 4b). This increase in CBF confirmed that baseline CBF was not maximal in either group and that modulation of the NO–cGMP pathway could increase CBF in healthy control subjects and in patients with PNTM. To further explore the NO–cGMP pathway, healthy control and PNTM patient cells were exposed to SNAP, a compound that releases NO upon photoactivation. SNAP addition led to a 30% increase in CBF in PNTM patient cells but no increase in CBF in cells from normal control subjects (Figure 4c).
To determine whether pharmacologic manipulation of the NO–cGMP pathway could improve CBF in PNTM cells, the selective inhibitor of cGMP-specific phosphodiesterase type 5, sildenafil, was used. A 40% increase from preexposure baseline CBF was seen in the PNTM patient cells, whereas the healthy control cells had no change in CBF. PNTM CBF was still significantly elevated compared with preexposure baseline at 8 hours (Figure 4d). The dose-response curve to sildenafil in PNTM patient cells had a broad plateau (Figure 4e). Although sildenafil increased PNTM cell resting CBF, it did not alter PNTM cell CBF response to TLR agonists (Figure 4f).
PNTM infection is not universal, even in diseases with well-known mucociliary clearance defects and pulmonary manifestations. In CF and PCD, the overall prevalence rate of PNTM infections is 11 to 13% (24, 25). This overall rate of PNTM infections is misleading because there is a striking increase seen in PNTM infections with increasing age. In patients with PCD, the PNTM rate is very low in children and increases to over 10% in adults over 30 years of age (24). In patients with CF who are over 40 years old, PNTM rates can reach 40% (25). The increasing prevalence of PNTM infection with increasing age is also seen in the general population (47). It is therefore very informative to study a population in which PNTM disease is a primary clinical manifestation.
Patients with PNTM disease have consistently low respiratory epithelial CBF (Figure 1a). The depressed baseline CBF is not associated with mycobacteria infection (Figures 1e and 2e; Figures E1d and E1e) or with factors associated with the respiratory epithelial cells (Figure 1b; Figures E1a and E1b) or secondary to inflammatory mediators produced by the patient’s immune system (Figures 1c and 1f). The depressed baseline CBF in patients with PNTM was unaffected by CFTR gene mutation status (Figure 1d), suggesting that the high rate of CFTR mutations in these patients does not cause their diminished CBF, even though it may contribute to disease susceptibility or severity in other ways.
These findings are important because they align PNTM disease with a group of diseases that have defects in mucociliary clearance, such as PCD and CF, and not chronic sinusitis (41). A deficiency in mucociliary clearance in patients with PNTM may also help to explain why the infection predisposition is specific to PNTM manifestations and why the patients do not present with disseminated NTM disease (48).
The critical role of TLRs in airway innate immunity is well established. We found abnormalities in the PNTM patient epithelial CBF responses to the TLR2, TLR3, TLR5, TLR7/8, and TLR9 agonists. A depressed CBF response to TLR agonists in PNTM epithelial cells was not present with all TLR agonists. CBF in response to the TLR4 agonist LPS was increased in PNTM respiratory epithelial cells when compared with healthy control respiratory cells (Figure 2d), suggesting that changes in the CBF responses to TLR agonists are unlikely to be due to mutations in the TLR signaling pathway, such as with the MyD88, IRAK4, or NEMO deficiencies (49). The CBF response to TLR agonists in an IRAK4-deficient subject, a protein that is required for all MyD88-dependent TLR signaling, showed no depression of baseline CBF, and the CBF response to TLR agonists was as expected. Changes in CBF in IRAK4-deficient respiratory epithelial cells were only seen in response to the TLR3 agonist poly(I:C) (Figure 2c). TLR3 signals through TRIF and not MyD88. This suggests that disruption of MyD88-dependent signaling could not account for the ex vivo phenotype observed in patients with PNTM. Additionally, cytokine production is similar between patients with PNTM and healthy control subjects.
We found a modest but significant decrease in nNO in patients with PNTM compared with healthy control subjects. The relative importance of this and its cause are under evaluation. The decrease in nNO did suggest that studying the NO–cGMP pathway might be informative. Administration of a cGMP analog, 8Br-cGMP, confirmed that CBF could be increased in patients with PNTM and control subjects. The swiftness of the response and the ability of both patient populations to respond to CBF indicated that the differences in CBF between patients with PNTM and healthy control subjects were unlikely to be due to altered cilia structure. Surprisingly, sildenafil exerted a selective CBF increase in PNTM patient cells ex vivo (Figure 4d). The normalization of CBF by sildenafil without normalization of PNTM patient TLR responses suggests that, although the ciliary and TLR functional defects may be etiologically linked, both responses do not normalize with NO augmentation, at least not at the doses and durations explored here.
Although we are still searching for the specific gene or genes responsible for this syndrome, decreased baseline CBF is a robust and consistent marker that offers a partial mechanistic basis for susceptibility to PNTM. The ability of NO donors to rapidly increase mucociliary activity in vivo has been demonstrated (44), indicating that modulation of the NO–cGMP pathway might be of therapeutic value. Furthermore, agents to supply or manipulate the NO–cGMP pathway are abundant, and increasing CBF may have direct clinical benefit.
These data suggest a defect in CBF in patients with PNTM that probably predisposes them to this infection. Identification of this defect in the respiratory epithelium may help to explain the lack of extrapulmonary infections in patients with PNTM and the previous failures to find robust immune dysfunction associated with the common entity of idiopathic PNTM. Familial clusters of PNTM disease are consistent with the hypothesis that this predisposition is genetically determined and likely precedes the development of PNTM infection. The existence of this predisposition is supported by the demonstration of a specific morphotype consisting of leanness and tallness that predates the onset of disease. The relative modesty of the abnormality we identify here aligns with the fact that most aspects of life are normal (e.g., development, fertility, activity) in patients with PNTM and that onset of PNTM disease is delayed until the sixth decade. The recognition of NO-cGMP pathway impairment in patients with PNTM offers immediate avenues for further mechanistic and therapeutic investigation.
The authors thank M. Knowles and M. Leigh for clinical evaluations, M. Zariwala for genetic analyses of some of the patients with PCD at the University of North Carolina as part of Rare Disease Clinical Research Network’s Genetic Disorders of Mucociliary Clearance Consortium, and S. Datta for providing invaluable assistance during manuscript preparation. NIAID imaging core provided imaging assistance.
1. | Taiwo B, Glassroth J. Nontuberculous mycobacterial lung diseases. Infect Dis Clin North Am 2010;24:769–789. |
2. | Tortoli E. Clinical manifestations of nontuberculous mycobacteria infections. Clin Microbiol Infect 2009;15:906–910. |
3. | Browne SK, Holland SM. Anticytokine autoantibodies in infectious diseases: pathogenesis and mechanisms. Lancet Infect Dis 2010;10:875–885. |
4. | Dorman SE, Holland SM. Interferon-gamma and interleukin-12 pathway defects and human disease. Cytokine Growth Factor Rev 2000;11:321–333. |
5. | Martín-Casabona N, Bahrmand AR, Bennedsen J, Thomsen VO, Curcio M, Fauville-Dufaux M, Feldman K, Havelkova M, Katila ML, Köksalan K, et al. Non-tuberculous mycobacteria: patterns of isolation. A multi-country retrospective survey. Int J Tuberc Lung Dis 2004;8:1186–1193. |
6. | Wang HX, Yue J, Han M, Yang JH, Gao RL, Jing LJ, Yang SS, Zhao YL. Nontuberculous mycobacteria: susceptibility pattern and prevalence rate in Shanghai from 2005 to 2008. Chin Med J (Engl) 2010;123:184–187. |
7. | Prevots DR, Shaw PA, Strickland D, Jackson LA, Raebel MA, Blosky MA, Montes de Oca R, Shea YR, Seitz AE, Holland SM, et al. Nontuberculous mycobacterial lung disease prevalence at four integrated health care delivery systems. Am J Respir Crit Care Med 2010;182:970–976. |
8. | Griffith DE. Nontuberculous mycobacterial lung disease. Curr Opin Infect Dis 2010;23:185–190. |
9. | Prince DS, Peterson DD, Steiner RM, Gottlieb JE, Scott R, Israel HL, Figueroa WG, Fish JE. Infection with Mycobacterium avium complex in patients without predisposing conditions. N Engl J Med 1989;321:863–868. |
10. | Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, Horsburgh R, Huitt G, Iademarco MF, et al.; ATS Mycobacterial Diseases Subcommittee; American Thoracic Society; Infectious Disease Society of America. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007;175:367–416. [Published erratum appears in Am J Respir Crit Care Med 2007;175:744–745.] |
11. | Reich JM, Johnson RE. Mycobacterium avium complex pulmonary disease presenting as an isolated lingular or middle lobe pattern: the Lady Windermere syndrome. Chest 1992;101:1605–1609. |
12. | Saleeb P, Olivier KN. Pulmonary nontuberculous mycobacterial disease: new insights into risk factors for susceptibility, epidemiology, and approaches to management in immunocompetent and immunocompromised patients. Curr Infect Dis Rep 2010;12:198–203. |
13. | Ziedalski TM, Kao PN, Henig NR, Jacobs SS, Ruoss SJ. Prospective analysis of cystic fibrosis transmembrane regulator mutations in adults with bronchiectasis or pulmonary nontuberculous mycobacterial infection. Chest 2006;130:995–1002. |
14. | Iseman MD, Buschman DL, Ackerson LM. Pectus excavatum and scoliosis: thoracic anomalies associated with pulmonary disease caused by Mycobacterium avium complex. Am Rev Respir Dis 1991;144:914–916. |
15. | Kim RD, Greenberg DE, Ehrmantraut ME, Guide SV, Ding L, Shea Y, Brown MR, Chernick M, Steagall WK, Glasgow CG, et al. Pulmonary nontuberculous mycobacterial disease: prospective study of a distinct preexisting syndrome. Am J Respir Crit Care Med 2008;178:1066–1074. |
16. | Dirac MA, Horan KL, Doody DR, Meschke JS, Park DR, Jackson LA, Weiss NS, Winthrop KL, Cangelosi GA. Environment or host? A case-control study of risk factors for Mycobacterium avium complex lung disease. Am J Respir Crit Care Med 2012;186:684–691. |
17. | Marras TK. Host susceptibility or environmental exposure in Mycobacterium avium complex lung disease: it takes two to tango. Am J Respir Crit Care Med 2012;186:585–586. |
18. | Colombo RE, Hill SC, Claypool RJ, Holland SM, Olivier KN. Familial clustering of pulmonary nontuberculous mycobacterial disease. Chest 2010;137:629–634. |
19. | Tanaka E, Kimoto T, Matsumoto H, Tsuyuguchi K, Suzuki K, Nagai S, Shimadzu M, Ishibatake H, Murayama T, Amitani R. Familial pulmonary Mycobacterium avium complex disease. Am J Respir Crit Care Med 2000;161:1643–1647. |
20. | Yamazaki Y, Kubo K, Sekiguchi M, Honda T. Analysis of BAL fluid in M. avium-intracellulare infection in individuals without predisposing lung disease. Eur Respir J 1998;11:1227–1231. |
21. | Al-Houqani M, Jamieson F, Mehta M, Chedore P, May K, Marras TK. Aging, COPD, and other risk factors do not explain the increased prevalence of pulmonary Mycobacterium avium complex in Ontario. Chest 2012;141:190–197. |
22. | Kwon YS, Kim EJ, Lee SH, Suh GY, Chung MP, Kim H, Kwon OJ, Koh WJ. Decreased cytokine production in patients with nontuberculous mycobacterial lung disease. Lung 2007;185:337–341. |
23. | Knowles MR, Boucher RC. Mucus clearance as a primary innate defense mechanism for mammalian airways. J Clin Invest 2002;109:571–577. |
24. | Noone PG, Leigh MW, Sannuti A, Minnix SL, Carson JL, Hazucha M, Zariwala MA, Knowles MR. Primary ciliary dyskinesia: diagnostic and phenotypic features. Am J Respir Crit Care Med 2004;169:459–467. |
25. | Olivier KN, Weber DJ, Wallace RJ Jr, Faiz AR, Lee JH, Zhang Y, Brown-Elliot BA, Handler A, Wilson RW, Schechter MS, et al.; Nontuberculous Mycobacteria in Cystic Fibrosis Study Group. Nontuberculous mycobacteria. I: multicenter prevalence study in cystic fibrosis. Am J Respir Crit Care Med 2003;167:828–834. |
26. | Teff Z, Priel Z, Gheber LA. The forces applied by cilia depend linearly on their frequency due to constant geometry of the effective stroke. Biophys J 2008;94:298–305. |
27. | Braiman A, Priel Z. Efficient mucociliary transport relies on efficient regulation of ciliary beating. Respir Physiol Neurobiol 2008;163:202–207. |
28. | Jiao J, Wang H, Lou W, Jin S, Fan E, Li Y, Han D, Zhang L. Regulation of ciliary beat frequency by the nitric oxide signaling pathway in mouse nasal and tracheal epithelial cells. Exp Cell Res 2011;317:2548–2553. |
29. | Lundberg JO, Weitzberg E. Nasal nitric oxide in man. Thorax 1999;54:947–952. |
30. | Fowler CJ, Colombo RE, Root H, Bryant CE, Olivier KN, Holland SM. Exploring PRR and ciliary function utilizing primary respiratory epithelial cell cultures. Presented at the Keystone Symposia, Innate Immunity at Mucosal Surfaces: The Players (Joint X5/X6), Feb 26 to Mar 3, 2011, Vancouver, BC, Canada. |
31. | Barbato A, Frischer T, Kuehni CE, Snijders D, Azevedo I, Baktai G, Bartoloni L, Eber E, Escribano A, Haarman E, et al. Primary ciliary dyskinesia: a consensus statement on diagnostic and treatment approaches in children. Eur Respir J 2009;34:1264–1276. |
32. | Parker D, Prince A. Innate immunity in the respiratory epithelium. Am J Respir Cell Mol Biol 2011;45:189–201. |
33. | Guillot L, Medjane S, Le-Barillec K, Balloy V, Danel C, Chignard M, Si-Tahar M. Response of human pulmonary epithelial cells to lipopolysaccharide involves Toll-like receptor 4 (TLR4)-dependent signaling pathways: evidence for an intracellular compartmentalization of TLR4. J Biol Chem 2004;279:2712–2718. |
34. | Sampaio EP, Elloumi HZ, Zelazny A, Ding L, Paulson ML, Sher A, Bafica AL, Shea YR, Holland SM. Mycobacterium abscessus and M. avium trigger Toll-like receptor 2 and distinct cytokine response in human cells. Am J Respir Cell Mol Biol 2008;39:431–439. |
35. | Kuhns DB, Long Priel DA, Gallin JI. Endotoxin and IL-1 hyporesponsiveness in a patient with recurrent bacterial infections. J Immunol 1997;158:3959–3964. |
36. | Lindberg S, Cervin A, Runer T. Low levels of nasal nitric oxide (NO) correlate to impaired mucociliary function in the upper airways. Acta Otolaryngol 1997;117:728–734. |
37. | Chilvers MA, Rutman A, O’Callaghan C. Ciliary beat pattern is associated with specific ultrastructural defects in primary ciliary dyskinesia. J Allergy Clin Immunol 2003;112:518–524. |
38. | Schmid A, Sutto Z, Schmid N, Novak L, Ivonnet P, Horvath G, Conner G, Fregien N, Salathe M. Decreased soluble adenylyl cyclase activity in cystic fibrosis is related to defective apical bicarbonate exchange and affects ciliary beat frequency regulation. J Biol Chem 2010;285:29998–30007. |
39. | Schipor I, Palmer JN, Cohen AS, Cohen NA. Quantification of ciliary beat frequency in sinonasal epithelial cells using differential interference contrast microscopy and high-speed digital video imaging. Am J Rhinol 2006;20:124–127. |
40. | Ho JC, Chan KN, Hu WH, Lam WK, Zheng L, Tipoe GL, Sun J, Leung R, Tsang KW. The effect of aging on nasal mucociliary clearance, beat frequency, and ultrastructure of respiratory cilia. Am J Respir Crit Care Med 2001;163:983–988. |
41. | Chen B, Antunes MB, Claire SE, Palmer JN, Chiu AG, Kennedy DW, Cohen NA. Reversal of chronic rhinosinusitis-associated sinonasal ciliary dysfunction. Am J Rhinol 2007;21:346–353. |
42. | Teff Z, Priel Z, Gheber LA. Forces applied by cilia measured on explants from mucociliary tissue. Biophys J 2007;92:1813–1823. |
43. | Rhee CS, Hong SK, Min YG, Lee CH, Lee KS, Ahn SH, Park KS, Yi WJ. Effects of IL-1 beta, TNF-alpha, and TGF-beta on ciliary beat frequency of human nasal ciliated epithelial cells in vitro. Am J Rhinol 1999;13:27–30. |
44. | Runer T, Lindberg S. Ciliostimulatory effects mediated by nitric oxide. Acta Otolaryngol 1999;119:821–825. |
45. | Imada M, Iwamoto J, Nonaka S, Kobayashi Y, Unno T. Measurement of nitric oxide in human nasal airway. Eur Respir J 1996;9:556–559. |
46. | Li D, Shirakami G, Zhan X, Johns RA. Regulation of ciliary beat frequency by the nitric oxide-cyclic guanosine monophosphate signaling pathway in rat airway epithelial cells. Am J Respir Cell Mol Biol 2000;23:175–181. |
47. | Adjemian J, Olivier KN, Seitz AE, Holland SM, Prevots DR. Prevalence of nontuberculous mycobacterial lung disease in U.S. Medicare beneficiaries. Am J Respir Crit Care Med 2012;185:881–886. |
48. | O’Connell ML, Birkenkamp KE, Kleiner DE, Folio LR, Holland SM, Olivier KN. Lung manifestations in an autopsy series of pulmonary or disseminated nontuberculous mycobacterial disease. Chest 2012;141:1203–1209. |
49. | Bustamante J, Boisson-Dupuis S, Jouanguy E, Picard C, Puel A, Abel L, Casanova JL. Novel primary immunodeficiencies revealed by the investigation of paediatric infectious diseases. Curr Opin Immunol 2008;20:39–48. |
This work was supported by National Institutes of Health grant 9U54HL096458-06 and by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases.
Author Contributions: C.J.F. designed the experiments, analyzed the data, and composed the manuscript. K.N.O. treated the patient population. J.M.L. extracted clinical values and treated the patient population. A.G.H., C.C.S., and H.R. coordinated patient recruitment. D.B.K. performed the cytokine assay. C.L. contributed tissue culture expertise. A.Z. contributed bacterial expertise. C.A.F. ensured Institutional Review Board compliance. J.D. and C.H. treated the patient population. J.H.S. provided critical input throughout the study. C.E.B. and S.M.H. were responsible for the overall design of the project and revision of the manuscript.
This article has an online supplement, which is accessible from this issue's table of contents at www.atsjournals.org
Originally Published in Press as DOI: 10.1164/rccm.201212-2197OC on April 17, 2013
Author disclosures are available with the text of this article at www.atsjournals.org.