Abstract
Because experimental studies have shown that intact alveolar epithelial fluid transport function is critical for resolution of pulmonary edema and acute lung injury, we measured net alveolar fluid clearance in 79 patients with acute lung injury or the acute respiratory distress syndrome. Pulmonary edema fluid and plasma were sampled serially in the first 4 hours after intubation. Net alveolar fluid clearance was calculated from sequential edema fluid protein measurements. Mean alveolar fluid clearance was 6%/h. Of the patients, 56% had impaired alveolar fluid clearance ( < 3%/h), 32% had submaximal clearance ( ⩾ 3%/h, < 14%/h), and 13% had maximal clearance ( ⩾ 14%/h). These findings are contrasted to our recent report of 65 patients with hydrostatic pulmonary edema, in whom mean alveolar fluid clearance was 13%/h; only 25% had impaired clearance whereas 75% had submaximal or maximal clearance (J Appl Physiol 1999;87:1301–1312). Acute lung injury with maximal alveolar fluid clearance were more likely to be female (p = 0.03), and less likely to have sepsis (p = 0.01). Endogenous and exogenous catecholamines did not correlate with alveolar fluid clearance. Patients with maximal alveolar fluid clearance had significantly lower mortality and a shorter duration of mechanical ventilation. In summary, in contrast to hydrostatic pulmonary edema, alveolar fluid clearance in patients with acute lung injury and the acute respiratory distress syndrome is impaired in the majority of patients, and maximal alveolar fluid clearance is associated with better clinical outcomes.
Acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS) are common causes of acute respiratory failure. Although much has been learned about the pathophysiology of ALI/ARDS, the resolution phase of ALI/ARDS is still poorly understood (1). Early ALI/ARDS is characterized by alveolar epithelial and lung endothelial injury leading to increased permeability pulmonary edema, alveolar filling, and respiratory failure. The reabsorption of pulmonary edema fluid from the alveolar space is necessary for the resolution of ALI/ARDS. A number of experimental studies have demonstrated that intact alveolar epithelial fluid transport function is critical for the resolution of experimental pulmonary edema and acute lung injury. However, little is known about the capacity of the injured alveolar epithelium to resolve pulmonary edema in patients with ALI/ARDS.
Alveolar fluid clearance has been measured in only a few clinical studies (2-4). The maximal capacity of the uninjured alveolar epithelium to clear pulmonary edema fluid has been characterized in a large study of patients with hydrostatic pulmonary edema (3). In that study, 75% of patients with severe hydrostatic pulmonary edema had intact alveolar fluid clearance (⩾ 3%/h). Furthermore, 38% of patients had alveolar fluid clearance rates more than twice the maximally stimulated rate measured in the ex vivo human lung (⩾ 14%/h), underscoring the importance of in vivo measurements. Previously, alveolar fluid clearance has been measured only in a small number of patients with ALI/ARDS. In contrast to patients with hydrostatic edema, 7 of 16 patients with ARDS were found to have no measurable alveolar fluid clearance, a finding that was associated with poorer oxygenation and increased mortality (2). However, alveolar fluid clearance has never been measured systematically in a large number of patients with ALI/ARDS.
This study was designed to examine alveolar fluid clearance in the setting of alveolar epithelial injury due to ALI/ ARDS. The first objective was to measure systematically the rate of net alveolar fluid clearance in a large number of patients with ALI/ARDS. Alveolar fluid clearance in this patient population was then compared with previously reported rates of alveolar fluid clearance in patients with hydrostatic pulmonary edema and no alveolar epithelial injury. The second objective was to study potential mechanisms that regulate alveolar fluid clearance in the setting of acute lung injury, including both catecholamine-dependent and -independent mechanisms. The third objective was to assess whether rapid alveolar fluid clearance is associated with a better prognosis in patients with ALI/ARDS.
Patients with ALI or ARDS as defined by the North American European Consensus Conference definitions (5) were identified from the adult intensive care units of Moffitt-Long Hospital, University of California (San Francisco, CA), and San Francisco General Hospital between 1985 and 1998. Additional inclusion criteria included acute respiratory failure requiring mechanical ventilation and aspirable pulmonary edema fluid within 1 h of tracheal intubation and repeated within 4 h. The initial edema fluid-to-plasma protein ratio was > 0.65, consistent with increased permeability pulmonary edema. The Committee for Human Research (University of California, San Francisco) approved the study.
Undiluted pulmonary edema fluid and plasma were collected simultaneously as previously described (2). All samples were centrifuged at 3,000 × g for 10 min and supernatants were stored at −70° C.
The total protein concentration in edema fluid and plasma was measured by the biuret method (2). If the sample volume was insufficient (< 1% of samples), then refractometry was used.
On the basis of the observation that the rate of clearance of edema fluid from the alveolar space is much faster than the rate of protein removal (6), the net alveolar fluid clearance rate was calculated: Percent alveolar fluid clearance = 100 × [1 − (initial edema protein/final edema protein)]. This method has been validated in prior clinical and experimental studies (2-4, 6-8). Patients were further categorized into three groups of alveolar fluid clearance on the basis of extrapolations from studies in the ex vivo human lung (3, 9): maximal = 14%/h; submaximal, ⩾ 3%/h and < 14%/h; or impaired, < 3%/h. For some analyses the submaximal and maximal clearance groups were combined into an intact clearance group.
Plasma epinephrine was measured by high-performance liquid chromatography (10). Because the effect of epinephrine on alveolar fluid clearance is short-lived (11), plasma epinephrine values determined at the beginning and the end of the interval over which alveolar fluid clearance was measured were averaged to estimate the mean plasma epinephrine level (3).
The primary etiology of ALI/ARDS was assessed on the basis of the clinical history. Sepsis was defined by standard criteria (12). Pneumonia was defined as new radiographic infiltrates and positive sputum cultures. Aspiration of gastric contents was defined as a witnessed aspiration event. Clinical data were recorded for the first 24 h after intubation and included hemodynamic parameters, respiratory and ventilatory parameters, multiorgan system function, demographic data, and medications administered. The Simplified Acute Physiology Score II (SAPS II) and the Lung Injury Score (LIS) were calculated (13, 14). Outcome variables included death before hospital discharge, change in alveolar–arterial oxygen difference at 4 and 24 h after enrollment, and days of unassisted ventilation during a 28-d period (15).
Continuous variables were compared by Students t test or by analysis of variance with the Student–Newman–Keuls test for multiple comparisons. Categorical variables were compared by using χ2 analysis. Nonparametric data were analyzed by using the Mann–Whitney U test or the Kruskal–Wallis test. Stepwise logistic regression was used to ascertain the independent predictors of (1) death and (2) greater than 21 d of unassisted ventilation. Statistical significance was defined as p ⩽ 0.05. All data are reported as means ± SD unless otherwise noted.
Over the study period, 116 patients with ALI or ARDS were identified and had two or more samples of pulmonary edema fluid aspirated. Thirty-seven of these patients were excluded from the analysis; 19 patients were excluded because either the first pulmonary edema fluid sample was not obtained within 1 h of endotracheal intubation, or the second sample was not obtained within 4 h of the first sample, and 18 patients were excluded because review of the medical record suggested a hydrostatic cause of pulmonary edema. The remaining 79 patients with ALI or ARDS were studied in the first 4 h after endotracheal intubation and mechanical ventilation. Demographic and clinical data are summarized in Table 1. The most common cause of ALI/ARDS was sepsis, including sepsis of both pulmonary and nonpulmonary origin. The mean initial edema fluid-to-plasma protein ratio was 0.98 ± 0.26, consistent with pulmonary edema due to increased permeability of the alveolar capillary barrier. Of note, the overall severity of illness was exceedingly high, as evidenced by the high SAPS II and the hospital mortality rate of 57%. The degree of pulmonary physiological impairment was also high, with a mean LIS of 2.9. Of the 79 patients, 7 patients met the definition of ALI; the remaining 72 met the definition of ARDS.
| Variable | Value* | |
|---|---|---|
| Age, yr | 45 ± 18 | |
| Male sex | 59% | |
| Race | ||
| White | 58% | |
| Asian/Pacific Islander | 20% | |
| African American | 12% | |
| Hispanic | 10% | |
| Present smoker | 29% | |
| Etiology of acute lung injury | ||
| Sepsis | 53% | |
| Drug overdose/drug reaction | 14% | |
| Pneumonia | 8% | |
| Aspiration of gastric contents | 8% | |
| Multiple transfusion | 6% | |
| Other | 11% | |
| SAPS II | 51 ± 19 | |
| Lung Injury Score | 2.9 ± 0.7 | |
| Hospital mortality | 57% |
Net alveolar fluid clearance was measured as the change in protein concentration between the first and last edema fluid samples obtained during the first 4 h after intubation and mechanical ventilation. The rate of alveolar fluid clearance ranged from a minimum of 0%/h (no net alveolar fluid clearance) to a maximum of 49%/h, with a mean of 6%/h. One additional patient had a rate of alveolar fluid clearance of 53% over 30 min, after which no further edema fluid samples could be aspirated. This patient was classified as having maximal alveolar fluid clearance.
A total of 44 patients (56%) had impaired alveolar fluid clearance (< 3%/h) whereas 35 patients (44%) had intact alveolar fluid clearance (⩾ 3%/h) (Figure 1). For some analyses, the group with intact alveolar fluid clearance was broken down into those with submaximal clearance (⩾ 3%/h, < 14%/h) and those with maximal clearance (⩾ 14%/h). The submaximal group included 32% of the patients (n = 25), with a mean rate of alveolar fluid clearance of 8 ± 3%/h (Figure 2). The maximal group included 13% of the patients (n = 10), with a mean rate of alveolar fluid clearance of 34 ± 28%/h.
Fig. 1. Plot of rate of alveolar fluid clearance in 79 patients with acute lung injury or the acute respiratory distress syndrome, showing that the majority of patients had impaired alveolar fluid clearance. Alveolar fluid clearance was measured from serial protein concentrations in the pulmonary edema fluid samples obtained from 79 patients. Intact clearance was defined as ⩾ 3%/h and impaired clearance as < 3%/ h. Each symbol (solid circle) represents the rate of alveolar fluid clearance in a single patient. n = number of subjects.
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Fig. 2. Percentage of patients with three categories of alveolar fluid clearance: impaired (< 3%/h), submaximal (⩾ 3%/h, < 14%/h), or maximal (⩾ 14%/h). Alveolar fluid clearance was measured during the first 4 h after intubation and mechanical ventilation in 79 patients with acute lung injury or the acute respiratory distress syndrome. Solid columns show the percentage of 79 patients in each group. N = number of subjects.
[More] [Minimize]To exclude a rise in plasma protein as a cause of net alveolar fluid clearance, plasma protein concentrations were compared at the beginning and end of each time interval over which alveolar fluid clearance was measured. Among the patients with impaired alveolar fluid clearance, plasma protein was 4.8 ± 0.8 g/dl at the first time point and 4.6 ± 1.0 g/dl at the second time point. Among the patients with submaximal alveolar fluid clearance, plasma protein was 5.1 ± 0.9 g/dl at the first time point and 5.0 ± 1.1 g/dl at the second time point. Among the patients with maximal alveolar fluid clearance, plasma protein was 5.6 ± 0.8 g/dl at the first time point and 5.6 ± 0.5 g/dl at the second time point. None of these differences were significant. Thus, changes in the intravascular protein osmotic pressure did not appear to influence the rate of net alveolar fluid clearance.
The association between selected clinical and demographic variables and the three categories of alveolar fluid clearance was evaluated by univariate analysis (Table 2). There was no significant association between demographic factors such as age or race and rate of alveolar fluid clearance. Interestingly, 8 of the 10 patients with maximal alveolar fluid clearance were female. By contrast, the majority of patients with submaximal or impaired alveolar fluid clearance were male, a significant difference (p = 0.03). Overall, mean alveolar fluid clearance was 9 ± 13%/h in females compared with 4 ± 6%/h in males (p < 0.01).
| Variable | Alveolar Fluid Clearance | p Value | ||||||
|---|---|---|---|---|---|---|---|---|
| Impaired (n = 44) | Submaximal (n = 25) | Maximal (n = 10) | ||||||
| N/Total (%) | ||||||||
| Male sex | 28/44 (64) | 16/25 (64) | 2/10 (20) | 0.03 | ||||
| Race, white/total | 22/41 (54) | 14/23 (61) | 7/10 (70) | 0.61 | ||||
| Present smoker | 9/38 (24) | 6/25 (24) | 1/9 (11) | 0.12 | ||||
| Sepsis | 22/44 (50) | 19/25 (76) | 2/10 (20) | 0.01 | ||||
| Mean ± SD | ||||||||
| Age, yr | 46 ± 18 | 45 ± 20 | 43 ± 12 | 0.10 | ||||
| Lung Injury Score | 2.9 ± 0.7 | 2.9 ± 0. | 2.7 ± 0.7 | 0.66 | ||||
| a–a oxygen difference | 481 ± 138 | 495 ± 133 | 411 ± 157 | 0.33 | ||||
| PaO2 /Fi O2 ratio | 114 ± 62 | 110 ± 67 | 131 ± 48 | 0.70 | ||||
| Tmax, degrees Celsius | 38.3 ± 0.9 | 38.2 ± 1.4 | 38.1 ± 0.7 | 0.82 | ||||
Although the number of current smokers was low, only one patient with maximal clearance was a smoker compared with 24% of the patients with submaximal or impaired clearance (p = 0.12). In addition, smokers had a higher initial edema fluid-to-plasma protein ratio, an index of alveolar capillary barrier permeability (median 1.03 versus 0.88 for nonsmokers, p < 0.05) and a trend toward more white blood cells in the pulmonary edema fluid (median 5.3 × 106/μl versus 1.5 = 106/μl in nonsmokers, p = 0.16). There was no association between body temperature or Lung Injury Score and rate of alveolar fluid clearance (Table 2).
A striking finding was that only 20% of patients with maximal alveolar fluid clearance had sepsis as the cause of ALI/ ARDS compared with 76% of the patients with submaximal alveolar fluid clearance and 50% of those with impaired clearance (p = 0.01). Overall, mean alveolar fluid clearance was twice as high in patients without sepsis, 8 ± 13%/h versus 4 ± 5%/h in patients with sepsis (p < 0.05). SAPS II also tended to be lower in the patients with maximal clearance, with a mean of 39 ± 10 compared with 55 ± 21 in the patients with submaximal clearance and 51 ± 19 in the patients with impaired clearance (p = 0.10).
A univariate analysis was done to test for an association between the rate of net alveolar fluid clearance and clinical outcomes including mortality, days of mechanical ventilation, and improvement in oxygenation. Overall, the group of patients with maximal alveolar fluid clearance had better clinical outcomes than patients with submaximal or impaired alveolar fluid clearance. Hospital mortality was 20% in patients with maximal clearance compared with 62% in patients with submaximal or impaired alveolar fluid clearance, a significant difference (Figure 3). In addition, the mean rate of alveolar fluid clearance was much higher in patients who survived compared with patients who died, 9 ± 13%/h in survivors versus 4 ± 4%/h in nonsurvivors, an almost 3-fold difference (p = 0.01). The median duration of unassisted ventilation in a 28-d period was significantly longer in patients with maximal alveolar fluid clearance, 23 versus 0 d in patients with submaximal or impaired alveolar fluid clearance (Figure 4). In addition, those patients who had a shorter duration of mechanical ventilation (⩾ 21 d of unassisted ventilation) had a mean alveolar fluid clearance of 11 ± 13%/h compared with 4 ± 8%/h in patients with a longer duration of mechanical ventilation (< 21 d of unassisted ventilation, p = 0.005). Finally, 100% of the patients with maximal alveolar fluid clearance had improved oxygenation at 24 h after study enrollment versus only 69% of patients with submaximal or impaired alveolar fluid clearance (p = 0.09). When just the first 4 h after enrollment were considered, patients with improved oxygenation had a mean alveolar fluid clearance rate of 7 ± 8%/h compared with patients with the same or worse oxygenation, who had a mean alveolar fluid clearance rate of 5 ± 10%/h (p = 0.04). In addition, the change in oxygenation over the first 4 h had a modest correlation with the rate of alveolar fluid clearance (r = 0.39, p = 0.001).
Fig. 3. Plot of hospital mortality of two groups of patients with acute lung injury or the acute respiratory distress syndrome: those with maximal alveolar fluid clearance (⩾ 14%/h) and those with impaired or submaximal alveolar fluid clearance (< 14%/h). Columns represent percent hospital mortality in each group. Hospital mortlality of patients with maximal alveolar fluid clearance was significantly less (p < 0.02). N = number of patients.
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Fig. 4. Box plot summary of days of unassisted ventilation in a 28-d period in two patient groups: those with maximal alveolar fluid clearance (⩾ 14%/h) and those with impaired or submaximal alveolar fluid clearance (< 14%/h). Each box encompasses 50% of the data (25th to 75th percentiles). Error bars encompass 80% of the data (10th to 90th percentiles). The thick horizontal lines represent median values. N = number of patients. Note that the duration of unassisted ventilation was significantly longer (*p < 0.005) in the patients with maximal alveolar fluid clearance, a better clinical outcome.
[More] [Minimize]When a multivariate analysis was done, only sepsis and a SAPS II ⩾ 50 predicted hospital mortality. Sepsis had an odds ratio of 7.7 (2.2–26.4) and SAPS II ⩾ 50 had an odds ratio of 3.6 (1.0–12.8). Similarly, only the absence of sepsis (odds ratio 0.47, 95% CI 0.24–0.94) or a SAPS II < 50 (odds ratio 0.35, 95% CI 0.15–0.81) predicted 21 or more days of unassisted ventilation.
To investigate a possible association between endogenous catecholamines and rate of alveolar fluid clearance, mean interval plasma epinephrine and norepinephrine levels were measured in a subset of 29 patients. Average interval epinephrine concentrations ranged from 0 to 75,674 pg/ml with a median of 124 pg/ ml. Average interval norepinephrine concentrations ranged from 0 to 118,546 pg/ml with a median of 689 pg/ml. Reported maximum plasma epinephrine concentrations in unstressed, supine, normotensive control human subjects are ∼ 100–150 pg/ml (16, 17). Reported maximum plasma norepinephrine concentrations in unstressed, supine, normotensive control human subjects are ∼ 250–400 pg/ml (16). There were no significant differences in the plasma epinephrine levels (Figure 5) or norepinephrine levels (data not shown) when compared with rates of alveolar fluid clearance. When epinephrine and norepinephrine levels were combined, or the administration of exogenous catecholamines (dobutamine or inhaled β-agonists) was considered along with endogenous epinephrine or norepinephrine levels, there was still no correlation with alveolar fluid clearance (data not shown). Furthermore, when individual patients were considered, it was clear that maximal alveolar fluid clearance could occur despite low levels of endogenous catecholamines, and conversely, that alveolar fluid clearance could be impaired despite high levels of endogenous catecholamines. For example, three patients with high plasma epinephrine levels (mean, 26,509 pg/ml) had no net alveolar fluid clearance, whereas two patients with maximal alveolar fluid clearance (mean, 36.2%/h) had no measurable plasma epinephrine. Pulmonary edema fluid levels of epinephrine and norepinephrine were measured in a small subset of patients and did not correlate with rate of alveolar fluid clearance (data not shown).
Fig. 5. Box plot summary of mean plasma epinephrine level in two groups of patients: those with impaired alveolar fluid clearance (< 3%/ h) and those with submaximal or maximal alveolar fluid clearance (⩾ 3%/ h). Each box encompasses 50% of the data (25th to 75th percentiles). Error bars encompass 80% of the data (10th to 90th percentiles). The thick horizontal lines represent median values. * Represents outliers. There was no significant difference between groups. N = number of patients; NS = not significant.
[More] [Minimize]Medications with potential effects on alveolar epithelial ion transport including diuretics (furosemide), exogenous glucocorticoids (methylprednisolone, hydrocortisone), inhaled β-agonists, (alupent, albuterol), and vasoactive agents (dobutamine, dopamine, epinephrine, norepinephrine, neosynephrine) were studied. There was no significant association between treatment with any of these agents and rate of alveolar fluid clearance.
Central venous pressure (Pcv) was measured in a total of 43 patients. There was no association between baseline Pcv, or decline in Pcv and the rate of alveolar fluid clearance. The number of patients with measurements of pulmonary arterial wedge pressure was too few for analysis. Patients with maximal alveolar fluid clearance were less likely to have shock in the first 24 h after study enrollment, although this difference did not reach significance (p = 0.20). Objective measurement of cardiac function by echocardiography within 48 h of pulmonary edema fluid sampling was obtained in 43 of the 79 patients. The mean ejection fraction was 56 ± 14%. The rate of alveolar fluid clearance was not significantly associated with ejection fraction.
Baseline indices of oxygenation in patients with different rates of alveolar fluid clearance are summarized in Table 2. The baseline alveolar–arterial oxygen difference, and the ratio of arterial partial pressure of oxygen to inspired oxygen fraction (PaO2 /Fi O2 ) were not different between the groups. The group of patients with maximal alveolar fluid clearance tended to have lower peak airway pressures by 6–7 cm H2O compared with the other groups, but this difference was not significant. There was also no significant difference in tidal volume or positive end-expiratory pressure (PEEP) among the three groups, although overall, the maximal clearance group had a lower mean PEEP and tidal volume per kilogram. Of the 10 patients with maximal alveolar fluid clearance, only 2 (20%) had a tidal volume greater than 12 ml/kg. By contrast, of the 65 patients with submaximal or impaired alveolar fluid clearance, 30 of 65 (46%) had a tidal volume greater than 12 ml/kg (p = 0.17).
Alveolar fluid clearance, an important function of the alveolar epithelium, has never been systematically characterized in a large group of patients with ALI or ARDS. The major findings of this study can be summarized as follows. First, net alveolar fluid clearance was impaired (< 3%/h) in the majority (56%) of patients. Maximal clearance (⩾ 14%/h) occurred in only 13% of patients, indicating that in the setting of clinical acute lung injury, only a minority of patients are able to upregulate alveolar fluid clearance. Second, females, nonsmokers, and patients without sepsis were more likely to have maximal alveolar fluid clearance. Furthermore, in this retrospective analysis, the rate of alveolar fluid clearance was not clearly associated with levels of endogenous or exogenous catecholamines. Third, the group of patients with maximal alveolar fluid clearance had better clinical outcomes, including lower hospital mortality, a shorter duration of mechanical ventilation, and a trend toward improved oxygenation at 24 h after endotracheal intubation.
The first objective was to characterize alveolar fluid clearance in a large number of patients with ALI or ARDS. The method used to quantify net alveolar fluid clearance was to measure serial protein concentrations in the pulmonary edema fluid, a modification of our validated experimental method used routinely by many investigators to quantify alveolar fluid clearance (6-8, 10, 11, 18-24). Are there other possible explanations for the increase in protein concentration in serial edema fluid samples? One possible explanation would be an increase in permeability of the alveolar capillary barrier causing an influx of edema fluid with a higher protein concentration. While it is conceivable that this occurred in some patients, even an increase to maximal permeability with alveolar flooding with pure plasma would fail to explain the rise in edema fluid protein to greater than simultaneous plasma protein in 25 of the 35 patients who had intact alveolar fluid clearance. A second possible explanation would be a rise in plasma protein over the sampling period leading to an influx of more protein-rich fluid into the alveoli, even with no change in alveolar capillary barrier permeability. However, we carefully quantified the changes in plasma protein over the period of edema fluid sampling and there was no rise in plasma protein over time (see Results). A third possible explanation would be the production of protein by cells in the airway or alveolar space, including inflammatory cells or epithelial cells. However, airway secretions have a low protein concentration (∼ 0.5 g/dl) (2) and thus tend to dilute rather than increase the total protein concentration. For these reasons, and because this and other clinical studies have shown correlations between alveolar fluid clearance and meaningful clinical indices of improvement in pulmonary edema such as improvement in chest radiograph and oxygenation (2-4), we believe that our method correctly estimates net alveolar fluid clearance in the majority of patients.
The most striking finding of our study was that alveolar fluid clearance was impaired (< 3%/h) in the majority of patients (56%). Only a minority of patients had maximal alveolar fluid clearance (⩾ 14%/h). Previously, alveolar fluid clearance has been measured only in a small group of 16 patients with ARDS (2). In that study, nearly half (7 of 16) of the patients had no net alveolar fluid clearance, a finding that was associated with poorer oxygenation and increased mortality. The current study has several advantages. First, the larger number of patients allows a rigorous analysis of factors associated with impaired or intact alveolar fluid clearance. Second, the current study quantifies the percent alveolar fluid clearance per hour, allowing differentiation of those patients with the most upregulated, maximal alveolar fluid clearance from those with slower, submaximal, or impaired alveolar fluid clearance. Third, clinical factors that might be associated with the rate of alveolar fluid clearance were more thoroughly analyzed.
We previously reported measurements of alveolar fluid clearance from 65 mechanically ventilated patients with severe hydrostatic pulmonary edema recruited over the same time period from the same intensive care units as in the current study (3). In that study, mean alveolar fluid clearance was 13%/h as compared with a mean alveolar fluid clearance of 6%/h in the current study of ALI/ARDS. Using the same classifications as in the current study, only a small minority of patients with hydrostatic pulmonary edema (25%) had impaired alveolar fluid clearance (< 3%/h) and more than one-third (38%) had maximal alveolar fluid clearance (⩾ 14%/h) (Figure 6). The patients with hydrostatic edema had a similar degree of pulmonary physiologic impairment and overall severity of illness as the patients with ALI/ARDS, with a comparable LIS (2.9 ± 0.6) and a high severity of illness as measured by SAPS II (45 ± 16). Thus, the rate of net alveolar fluid clearance is independent of the amount of pulmonary edema as measured by the LIS, and the cause of pulmonary edema is an important determinant of the rate of resolution. Patients with hydrostatic pulmonary edema are far more likely to have preserved or upregulated alveolar fluid clearance than those with ALI/ARDS. This difference is probably due to preservation of an intact and functional alveolar epithelial barrier in the majority of patients with hydrostatic edema.
Fig. 6. Comparison of rates of alveolar fluid clearance in two groups of patients: 65 mechanically ventilated patients with severe hydrostatic pulmonary edema and 79 mechanically ventilated patients with acute lung injury or the acute respiratory distress syndrome. Columns represent the percentage of patients in each group with three categories of alveolar fluid clearance: impaired (< 3%/h), submaximal (⩾ 3%/h, < 14%/h), or maximal (⩾ 14%/h). Data on patients with hydrostatic pulmonary edema reproduced with permission from Verghese GM, Ware LB, Matthay BA, Matthay MA. J Appl Physiol 1999;87:1301–1312.
[More] [Minimize]Eighty percent of the patients with maximal alveolar fluid clearance were female as opposed to only 35% of the patients with impaired or submaximal clearance (p = 0.03). Furthermore, the majority (seven of eight) of the female patients with maximal alveolar fluid clearance were less than 55 yr old, suggesting that they were premenopausal. This is an intriguing observation because experimental data indicate that in rats, exposure to estrogen and progesterone increases both the expression and function of the epithelial sodium channel (25). Cigarette smoking has never been associated with adverse outcomes in ALI/ARDS (12, 26). Although the number of smokers in our study was small (n = 20), there was a trend toward fewer smokers in the maximal alveolar fluid clearance group (p = 0.10), suggesting that cigarette smoking may have had an adverse effect on alveolar fluid clearance. In addition, smokers had a higher initial edema fluid-to-plasma protein ratio, an index of alveolar capillary barrier permeability, and tended to have more white blood cells in the pulmonary edema fluid. Although the effects of smoking on alveolar epithelial fluid transport have not been investigated experimentally, cigarette smoking has been shown to cause increased alveolar epithelial permeability (27), intraalveolar accumulation of neutrophils, and intraalveolar oxidant stress (28, 29).
In addition to sex and smoking status, the cause of ALI/ ARDS was an important determinant of alveolar fluid clearance. As shown in Figure 7, patients with sepsis were significantly less likely to have maximal alveolar fluid clearance. The most likely explanation is that sepsis was associated with more severe and lasting injury to the alveolar capillary barrier than other causes of lung injury, such as aspiration of gastric contents or primary pneumonia. In classic ultrastructural studies by Bachofen and Weibel (30), sepsis-associated lung injury was characterized by necrosis and sloughing of the alveolar epithelial barrier. This hypothesis is also supported by our experimental study of sheep (31), in which intravenous infusion of Pseudomonas aeruginosa for 8 h led to severe alveolar epithelial injury and abolished alveolar epithelial fluid transport function in 30% of the sheep. However, not all experimental models of sepsis are associated with impaired alveolar fluid clearance. In a short-term, 4-h rat model of bacteremia, alveolar fluid clearance was increased because of increased levels of endogenous catecholamines (10).
Fig. 7. Plot of percentage of patients with maximal alveolar fluid clearance (⩾ 14%/h) in two groups of patients with acute lung injury or the acute respiratory distress syndrome: those with no evidence of sepsis, and those with sepsis. The percentage of septic patients with maximal alveolar fluid clearance was significantly less (p = 0.02) than that of nonseptic patients.
[More] [Minimize]Catecholamines upregulate alveolar fluid clearance in both the normal and the injured lung in a variety of species, including the human lung. Endogenous epinephrine has been shown to upregulate alveolar fluid clearance under several experimental conditions including hypovolemic and septic shock in rats (10, 32), and neurogenic pulmonary edema in dogs (21). A variety of exogenous β2-agonists, including terbutaline, salmeterol, epinephrine, and dobutamine, can also stimulate alveolar fluid clearance (6, 20, 33-37). Although β2-agonists were the first to be characterized, isoproterenol, a β1- and β2-agonist, has been shown to upregulate alveolar fluid clearance by a β1-dependent mechanism (22). Therefore, we carefully quantified both endogenous and exogenous catecholamines including both β1- and β2-agonists.
Interestingly, levels of endogenous catecholamines did not correlate with alveolar fluid clearance (Figure 5). The administration of exogenous catecholamines also did not correlate with alveolar fluid clearance. Furthermore, when the combination of either high levels of endogenous catecholamines or the administration of exogenous catecholamines was considered, there was still no association with rate of alveolar fluid clearance. Why did endogenous and exogenous catecholamines fail to increase alveolar fluid clearance? There are several possible explanations. First, the analysis of catecholamine levels was done retrospectively and thus was not designed to test the hypothesis in a prospective, randomized fashion. Second, in some patients, injury to the alveolar epithelium may have rendered it unable to respond to catecholamine stimulation (38, 39). Third, the presence of high levels of endogenous or exogenous catecholamines over a prolonged period may have led to β-agonist receptor downregulation. Fourth, the majority of patients may not have received a sufficient or sustained exposure to alveolar β-agonists; only 30% of the patients received an inhaled β-agonist, and many of these received only one dose. Randomized trials of inhaled β-agonists are needed to definitively answer the question of whether patients with ALI/ARDS can increase alveolar fluid clearance in response to an adequate intraalveolar concentration of β-agonist.
In addition to catecholamines, several other pharmacological agents can increase alveolar fluid clearance. Dopamine increases alveolar fluid clearance by stimulation of the dopamine 1 receptor (23, 24, 40). However, we found no association between administration of dopamine and rate of alveolar fluid clearance. Glucocorticoids also enhance alveolar fluid clearance experimentally (41), but there was no association between administration of glucocorticoids and alveolar fluid clearance. An additional catecholamine-independent mechanism that might be important in determining the rate of alveolar fluid clearance is alveolar epithelial Type II cell hyperplasia (42, 43).
This study establishes that rapid alveolar fluid clearance is associated with improved clinical outcomes in patients with ALI/ARDS. Maximal alveolar fluid clearance was associated with decreased mortality, shorter duration of mechanical ventilation, and a trend toward improved oxygenation at 24 h after study enrollment. These findings lend support to the hypothesis that the resolution phase of ALI/ARDS is an important determinant of outcome. However, when a multivariate analysis was done, only sepsis and SAPS II were independent predictors of death or prolonged mechanical ventilation. One explanation is that sepsis is such a strong predictor of poor outcome in this and other studies (12, 26, 44). When only patients without sepsis were further analyzed by logistic regression, SAPS II continued to predict mortality and alveolar fluid clearance entered the regression with a p value of 0.12. A second explanation is that impaired alveolar fluid clearance and sepsis are closely associated variables. In fact, the association of sepsis with an inability to upregulate alveolar fluid clearance may, in part, explain why sepsis is associated with increased mortality from ALI/ARDS.
A limitation of our study is that because it was confined to those patients that could have serial samples of pulmonary edema fluid aspirated, the results are generalizable only to this population. However, we believe that our study population represented ALI/ARDS patients from our institution remarkably well. Comparing our patient population with a prospective study of 123 consecutive patients with ALI/ARDS admitted to our institution (12), mortality was similar (58 versus 57% in the current study, with a mean age of 52 (versus 45 in current study) and a similar incidence of sepsis (41 versus 53% in the current study).
In conclusion, this study has several important new findings. Alveolar fluid clearance was impaired in the majority of patients with clinical acute lung injury, in contrast to our previous findings in patients with pulmonary edema from hydrostatic causes. Because maximal alveolar fluid clearance was associated with improved clinical outcomes including lower mortality and a shorter duration of mechanical ventilation, future investigations should focus on the mechanisms for impaired alveolar fluid clearance in ALI/ARDS. As emphasized in editorials and reviews (45-47), new therapies targeted at enhancing alveolar fluid clearance may hasten the resolution of ALI/ARDS and improve survival.
The authors thank Brian Daniel, RRT, Rich Kallet, RRT, and Thomas Nuckton, M.D., for assisting with the collection of pulmonary edema fluid samples.
Supported by NIH HL 51856.
| 1. | Matthay MAConference summary: acute lung injury. Chest1161999119S126S |
| 2. | Matthay MA, Wiener-Kronish JPIntact epithelial barrier function is critical for the resolution of alveolar edema in humans. Am Rev Respir Dis142199012501257 |
| 3. | Verghese GM, Ware LB, Matthay BA, Matthay MAAlveolar epithelial fluid transport and the resolution of clinically severe hydrostatic pulmonary edema. J Appl Physiol87199913011312 |
| 4. | Ware LB, Golden JA, Finkbeiner WE, Matthay MAAlveolar epithelial fluid transport capacity in reperfusion lung injury after lung transplantation. Am J Respir Crit Care Med1591999980988 |
| 5. | Bernard GR, Artigas A, Brigham KL, Carlet J, Falke K, Hudson L, Lamy M, Legall JR, Morris A, Spragg R, Consensus Committee. The American–European Consensus Conference on ARDS: definitions, mechanisms, relevant outcomes, and clinical trial coordination. Am J Respir Crit Care Med 1994;149:818–824. |
| 6. | Berthiaume Y, Staub NC, Matthay MABeta-adrenergic agonists increase lung liquid clearance in anesthetized sheep. J Clin Invest791987335343 |
| 7. | Jayr C, Garat C, Meignan M, Pittet JF, Zelter M, Matthay MAAlveolar liquid and protein clearance in anesthetized, ventilated rats. J Appl Physiol76199426262642 |
| 8. | Matthay MA, Folkesson HG, Verkman ASSalt and water transport across alveolar and distal airway epithelia in the adult lung. Am J Physiol2701996L487L503 |
| 9. | Sakuma T, Okinawa G, Nakada T, Fujimura S, Matthay MAAlveolar fluid clearance in the resected human lung. Am J Respir Crit Care Med1501994305310 |
| 10. | Pittet JF, Wiener-Kronish JP, McElroy MC, Folkesson HG, Matthay MAStimulation of lung epithelial liquid clearance by endogenous release of catecholamines in septic shock in anesthetized rats. J Clin Invest941994663671 |
| 11. | Charron PD, Fawley JP, Maron MBEffect of epinephrine on alveolar liquid clearance in the rat. J Appl Physiol871999611618 |
| 12. | Doyle RL, Szaflarski N, Modin GW, Wiener-Kronish JP, Matthay MAIdentification of patients with acute lung injury: predictors of mortality. Am J Respir Crit Care Med152199518181824 |
| 13. | LeGall J, Lemshow S, Saulnier FA new Simplified Acute Physiology Score (SAPS II) based on a European/North American multicenter study. JAMA270199329572963 |
| 14. | Murray JF, Matthay MA, Luce JM, Flick MRAn expanded definition of the adult respiratory distress syndrome. Am Rev Respir Dis1381988720723 |
| 15. | Artigas A, Bernard GR, Carlet J, Dreyfuss D, Gattinoni L, Hudson L, Lamy M, Marini JJ, Matthay MA, Pinsky MR, Spragg R, Suter PM, Consensus Committee. The American–European Consensus Conference on ARDS: 2. Ventilatory, pharmacologic, supportive therapy, study design strategies and issues related to recovery and remodeling. Intensive Care Med 1998;24:378–398. |
| 16. | Cryer PEPhysiology and pathophysiology of the human sympathoadrenal system. N Engl J Med3031980436444 |
| 17. | Rosano T, Swift T, Hayes LAdvances in catecholamine and metabolite measurements for diagnosis of pheochromocytoma. Clin Chem37199118541867 |
| 18. | Matthay MA, Landolt CC, Staub NCDifferential liquid and protein clearance from the alveoli of anesthetized sheep. J Appl Physiol53198296104 |
| 19. | Matthay MA, Berthiaume Y, Staub NCLong-term clearance of liquid and protein from the lungs of unanesthetized sheep. J Appl Physiol591985928934 |
| 20. | Sakuma T, Folkesson HG, Suzuki S, Okaniwa G, Fujimura S, Matthay MABeta-adrenergic agonist stimulated alveolar fluid clearance in ex vivo human and rat lungs. Am J Respir Crit Care Med1551997506512 |
| 21. | Lane SM, Maender KC, Awender NE, Maron MBAdrenal epinephrine increased alveolar liquid clearance in neurogenic pulmonary edema. Am J Respir Crit Care Med1581998760768 |
| 22. | Norlin A, Finley N, Abedinpour P, Folkesson HGAlveolar liquid clearance in the guinea pig. Am J Physiol2741998L235L243 |
| 23. | Saldias FJ, Lecuona E, Comellas AP, Ridge KM, Sznajder JIDopamine restores lung ability to clear edema in rats exposed to hyperoxia. Am J Respir Crit Care Med1591999626633 |
| 24. | Barnard M, Olivera W, Rutschman D, Bertorello A, Katz A, Sznajder JDopamine stimulates sodium transport and liquid clearance in rat lung epithelium. Am J Respir Crit Care Med1561997709714 |
| 25. | Sweezey N, Tchepichev S, Gagnon S, Fertuck K, O'Brodovich HFemale gender hormones regulate mRNA levels and function of the rat lung epithelial Na channel. Am J Physiol2741998C379C386 |
| 26. | Zilberberg MD, Epstein SKAcute lung injury in the medical ICU. Comorbid conditions, age, etiology and hospital outcome. Am J Respir Crit Care Med157199811591164 |
| 27. | Jones JG, Minty BD, Lawler P, Hulands G, Crawley JCW, Veall NIncreased alveolar epithelial permeability in cigarette smokers. Lancet119806668 |
| 28. | Li XY, Rahman I, Donaldson K, MacNee WMechanisms of cigarette smoke induced increased airspace permeability. Thorax511996465471 |
| 29. | Morrison D, Rahman I, Lannan S, MacNee WEpithelial permeability, inflammation and oxidant stress in the air spaces of smokers. Am J Respir Crit Care Med1591999473479 |
| 30. | Bachofen ME, Weibel ERAlterations of the gas exchange apparatus in adult respiratory insufficiency associated with septicemia. Am Rev Respir Dis1161977589615 |
| 31. | Pittet JF, Wiener-Kronish JP, Serikov V, Matthay MAResistance of the alveolar epithelium to injury from septic shock in sheep. Am J Respir Crit Care Med151199510931100 |
| 32. | Modelska K, Matthay MA, McElroy MC, Pittet JFUpregulation of alveolar liquid clearance after fluid resuscitation for hemorrhagic shock in rats. Am J Physiol2761997L844L857 |
| 33. | Berthiaume Y, Broaddus VC, Gropper MA, Tanita T, Matthay MAAlveolar liquid and protein clearance from normal dog lungs. J Appl Physiol651988585593 |
| 34. | Crandall ED, Heming TA, Palombo RL, Goodman BEEffects of terbutaline on sodium transport in isolated perfused rat lung. J Appl Physiol601986289294 |
| 35. | Maron MBDose–response relationship between plasma epinephrine concentration and alveolar liquid clearance in dogs. J Appl Physiol85199817021707 |
| 36. | Tibayan F, Chesnutt A, Folkesson H, Eandi J, Matthay MDobutamine increases alveolar liquid clearance in ventilated rats by beta-2 receptor stimulation. Am J Respir Crit Care Med1561997438444 |
| 37. | Lasnier JM, Wangensteen OD, Schmitz LS, Gross CR, Ingbar DHTerbutaline stimulates alveolar fluid resorption in hyperoxic lung injury. J Appl Physiol81199617831789 |
| 38. | Guo Y, Duvall D, Crow JP, Matalon SNitric oxide inhibits Na absorption across cultures of alveolar type II monolayers. Am J Physiol2741998L369L377 |
| 39. | Modelska K, Matthay MA, Brown LAS, Deutsch E, Lu L, Pittet JFInhibition of beta-adrenergic dependent alveolar epithelial clearance by oxidant mechanisms after hemorrhagic shock in rats. Am J Physiol2761999L844L857 |
| 40. | Barnard ML, Ridge KM, Saldias F, Gare M, Friedman E, Guerrero C, Lecuona E, Bertorello AM, Katz AI, Sznajder JIStimulation of the dopamine 1 receptor increases lung edema clearance. Am J Respir Crit Care Med1601999982986 |
| 41. | Folkesson HG, Norlin A, Wang Y, Abedinpour P, Matthay MADexamethasone and thyroid hormone pretreatment upregulate alveolar epithelial fluid clearance in adult rats. J Appl Physiol882000416422 |
| 42. | Folkesson HG, Nitenberg G, Oliver BL, Jayr C, Albertine KH, Matthay MAUpregulation of alveolar epithelial fluid transport after subacute lung injury in rats from bleomycin. Am J Physiol2751998L478L490 |
| 43. | Wang Y, Folkesson HG, Jayr C, Ware LB, Matthay MAAlveolar epithelial fluid transport can be simultaneously upregulated by both KGF and beta-agonist therapy. J Appl Physiol87199918521860 |
| 44. | Monchi M, Bellenfant F, Cariou A, Joly L-M, Thebert D, Laurent I, Dhainaut J-F, Brunet FEarly predictive factors of survival in the acute respiratory distress syndrome: a multivariate analysis. Am J Respir Crit Care Med158199810761081 |
| 45. | Berthiaume Y, Lesur O, Dagenais ATreatment of acute respiratory distress syndrome: plea for rescue therapy of the alveolar epithelium. Thorax541999150160 |
| 46. | Sznajder JIStrategies to increase the alveolar epithelial fluid removal in the the injured lung. Am J Respir Crit Care Med160199914411442 |
| 47. | Ware LB, Matthay MAMedical progress: the acute respiratory distress syndrome. N Engl J Med342200013341349 |
(Received in original form April 7, 2000 and in revised form November 21, 2000)
